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+ Pharma
+ Stock solution
+ Antibiotics
+ Ampicillin
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Ken Kyagra

How can i prepare ampicillin and kanamycin stock solution?

Omme Salma   Follow

You got to prepare stock solutions of Ampicillin (100 mg/mL) and Kanamycin (50 mg/mL). Then add 1 uL of Ampicilln or Kansmycin in 1 mL medium (it will be 1:1000 diluted). Working concentration of the Ampicillin and Kanamycin is 50-100 and 25-50 ug/mL respectively.

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Bengt Nyman  Follow

Thanks a lot
now the idea is clear for me .

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Alan Humason  Follow

If you’ll be preparing plates with a final concentration of 100 ug/mL ampicillin, you should make a stock solution of 100,000 ug/mL (100 mg/mL).

1. Mix 1 g Amp powder into 10 mL of d H2O.
2. Vortex until powder is thoroughly dissolved.
3. Sterilize using 0.22-μm sterile filter.
4. Aliquot 1 mL of Amp solution per 1.5 mL tube and store at -20 °C.

Dilute your antibiotic into your LB medium at 1:1,000. For example, to make 100 mL of LB/ampicillin growth media, add 100 μL of a 100 mg/mL ampicillin stock.


  • Ampicillin : add 1ml ampicillin (at 100mg/ml) per liter of agar to obtain a final concentration of 100ug/ml.
    • Kanamycin : add 1ml kanamycin stock (at 50mg/ml) per liter of agar to obtain a final concentration of 50ug/ml.
    • Tetracycline : add 1ml tetracycline stock (at 15mg/ml) per liter of agar to obtain a final concentration of 15ug/ml.
    • Chloramphenicol :add 1ml chloramphenicol stock (at 25mg/ml) per liter of agar to obtain a final concentration of 100ug/ml.

  • How to determine volumes to use to obtain a certain concentration:
Use the following formula to determine amount of stock solution to use for any amount.

C1V1 = C2V2
C = concentration; V = volume

Example: You have a stock solution of A at 100mg/ml. You want 150 ml at 100ug/ml. Use the C1V1 for what you want, and the C2V2 for what you have.
Just plug in the numbers:

(100ug/ml)(150ml) = (100000ug/ml)(x)
15000 = 100000x
x = 0.15ml, or 150ul

Remember that you have to convert mg/ml to ug/ml, which you can do by multiplying by 1000.





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