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Measurement of sodium alginate molecular weight
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Matthieu d'Argencé
Measurement of sodium alginate molecular weight
(1) Sample preparation.Use an electronic scale to collect 20 mg of sodium alginate powder to dissolve in 10 ml of sodium nitrate solution, and stir on a magnetic mixer for two hours. (2) Sample filtering.Use a disposable sterile syringe and an organic syringe filter to filter the sample used. (3) Test.Open the gel penetration chromatography -optical scattering linked use instrument test software, zero, and the baseline stabilize, set the sample concentration to 2 mg/ml, use gel penetration chromatography -light scattering firing instrument with a syringe, absorb 200 200The solution after μL is filtered (guaranteed the internal bubbles inside), inserted the syringe needle into the instrument, slowly pushed the solution into, and the instrument was turned off in the sample valve to test the sample.Wash the syringe with sodium nitrate every time the same quality is performed.Chromatographic conditions are: chromatography column: G5000PWX1; flow phase nano3, concentration of 0.1 m/L; flow velocity 1 ml/min; column temperature is 35 ° C; sample volume is 100.0 μL.
(1) Sample preparation.Use an electronic scale to collect 20 mg of sodium alginate powder to dissolve in 10 ml of sodium nitrate solution, and stir on a magnetic mixer for two hours. (2) Sample filtering.Use a disposable sterile syringe and an organic syringe filter to filter the sample used. (3) Test.Open the gel penetration chromatography -optical scattering linked use instrument test software, zero, and the baseline stabilize, set the sample concentration to 2 mg/ml, use gel penetration chromatography -light scattering firing instrument with a syringe, absorb 200 200The solution after μL is filtered (guaranteed the internal bubbles inside), inserted the syringe needle into the instrument, slowly pushed the solution into, and the instrument was turned off in the sample valve to test the sample.Wash the syringe with sodium nitrate every time the same quality is performed.Chromatographic conditions are: chromatography column: G5000PWX1; flow phase nano3, concentration of 0.1 m/L; flow velocity 1 ml/min; column temperature is 35 ° C; sample volume is 100.0 μL.
(2) Sample filtering.Use a disposable sterile syringe and an organic syringe filter to filter the sample used.
(3) Test.Open the gel penetration chromatography -optical scattering linked use instrument test software, zero, and the baseline stabilize, set the sample concentration to 2 mg/ml, use gel penetration chromatography -light scattering firing instrument with a syringe, absorb 200 200The solution after μL is filtered (guaranteed the internal bubbles inside), inserted the syringe needle into the instrument, slowly pushed the solution into, and the instrument was turned off in the sample valve to test the sample.Wash the syringe with sodium nitrate every time the same quality is performed.Chromatographic conditions are: chromatography column: G5000PWX1; flow phase nano3, concentration of 0.1 m/L; flow velocity 1 ml/min; column temperature is 35 ° C; sample volume is 100.0 μL.
(2) Sample filtering.Use a disposable sterile syringe and an organic syringe filter to filter the sample used.
(3) Test.Open the gel penetration chromatography -optical scattering linked use instrument test software, zero, and the baseline stabilize, set the sample concentration to 2 mg/ml, use gel penetration chromatography -light scattering firing instrument with a syringe, absorb 200 200The solution after μL is filtered (guaranteed the internal bubbles inside), inserted the syringe needle into the instrument, slowly pushed the solution into, and the instrument was turned off in the sample valve to test the sample.Wash the syringe with sodium nitrate every time the same quality is performed.Chromatographic conditions are: chromatography column: G5000PWX1; flow phase nano3, concentration of 0.1 m/L; flow velocity 1 ml/min; column temperature is 35 ° C; sample volume is 100.0 μL.
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