Old problem with carboxylated particles EDC / NHS coupling protein

Hello landlord, please ask the carboxylated particles why the EDC / NHS reaction is carried out, the protein has an amino-based end

Try with MES buffer

I am also plagued in this question, do you find the result now?

I am also like this

I didn't make it, I feel that many people can make it

EDC overhang caused microsphere reunion

Have you done it?I also encountered a similar problem, thank you, I hope to explore thank you