Human Prothrombin
9001-26-7
95%
2mg
2026-07-05
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Human Prothrombin Cat No CZY-019 Description Prothrombin is a vitamin K-dependent plasma protein which is synthesized in the liver. Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to γ-carboxyglutamic acid (gla). The ten gla residues are located within the first 40 amino acids of the mature protein and contribute to the ability of prothrombin to bind to negatively charged phospholipid membranes. Prothrombin contains two regions of internal homology which are referred to as "kringle" structures. These regions of conspicuous secondary structure are located between residues 40 and 270 of the mature plasma protein and replace the growth factor domains found in several other plasma serine proteases. Thus far, no function has been ascribed to these regions, but there is suspicion that they may play a role in one of several binary protein interactions involving prothrombin. The mature single chain protein circulates in plasma as a zymogen and, during coagulation, is proteolytically activated to the potent serine protease α-thrombin. This proteolysis is catalyzed by the prothrombinase enzyme complex. During activation, prothrombin is cleaved at Arg271-Thr272 (human) / Arg273-Thr274 (bovine) and at Arg320-Ser321 (human) / Arg323-Ser324 (bovine) to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. In the case of human prothrombin/thrombin, there is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.Human prothrombin is prepared from fresh frozen human plasma as described by Bajaj and coworkers. Bovine prothrombin is prepared from fresh bovine plasma using a modification of the procedure described by Owen and coworkers. Purified prothrombin is supplied in 50% (vol/vol) glycerol/H2O and should be stored at -20°C. Purity is determined by SDS-PAGE analysis, and activity is measured by clotting and/or chromogenic substrate assay, following conversion of prothrombin to thrombin. Source Human Package 2 mg CAS No 9001-26-7 Molecular Weight 72000 Purity >95% by SDS-PAGE Concentration 13.7 mg/mL Isoelectric point 4.7-4.9 Structure single chain, NH2-terminal gla domain, two kringle regions Stability 12 months Storage -20°C Buffer 50% Glycerol/ H2O (vol/vol) Formulation 50% glycerol/water (v/v) Localization Plasma Specific Activity 9.7 U/mg Extinction coefficient 13.8 Percent carbohydrate 0.082 Post-translational modifications ten gla residues category Specialty Chemicals(c26) cas_num 9001-26-7 Basic Info-
Product Name:
Prothrombin
Other Name:Blood-coagulation factor II;Prothrombin;Prothrombase;Blood-coagulating factor II;Blood-coagulation factor 2;Factor II;Blood clotting factor II;Prothrombin complex;Coagulation factor II;DRF;Differentiation reversal factor;Growth factors (animal),DRF (differentiation reversal factor);Gene F2 protein;Coagulation factor 2;Blood clotting factor 2;Prothrombins;F2;9023-22-7;9035-57-8
CAS No.:9001-26-7
Molecular Weight:1355.365
Exact Mass:1354.567383
Categories:
CharacteristicsHazard IdentificationClassification of the substance or mixture
no data available
GHS label elements, including precautionary statements
Pictogram(s) no data available Signal word no data available
Hazard statement(s) no data available
Precautionary statement(s) Prevention no data available
Response no data available
Storage no data available
Disposal no data available
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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