5-Amino-1-methylquinolinium chloride CAS No.:42464-96-0 98.0% purity min.

1.Product introduction

The cytosolic enzyme nicotinamide N-methyltransferase (NNMT) has been newly discovered to regulate the levels of nicotinamide precursors required for NAD+ biosynthesis and therefore plays a crucial role in regulating the NAD+ salvage pathway and cellular metabolism. NNMT is expressed in skeletal muscle, and its expression gradually increases with aging of muscle tissue. Importantly, NNMT is a major component of the sarcopenic gene expression signature, and its overexpression may lead to a significant decrease in NAD+ levels and the decline in muscle mass, strength, and function that accompanies aging. Treatment of aged mice with a small molecule NNMT inhibitor (NNMTi) enhanced the proliferation, fusion, and regeneration capacity of muSCs, thereby improving the functional performance of skeletal muscle. Similar results were obtained in vitro using C2C12 cells, which are very similar to muSCs, where NNMTi treatment promoted myoblast differentiation and supported metabolic changes in cellular metabolites associated with the NAD + salvage pathway.

NNMTi is an enzyme overexpressed during skeletal muscle aging and is associated with damage to the NAD+ pathway, dysregulated sirtuin1 activity, and increased muscle stem cell senescence. NNMT is a metabolic enzyme that catalyzes the methylation of nicotinamide (NAM) using the universal methyl donor S-adenosylmethionine (SAM), linking the metabolic state of the cell to the methylation balance and intracellular NAD+ levels. NNMT is abundantly expressed in the liver and adipose tissue, as well as in pathological conditions characterized by increased metabolic demands, such as certain fatty liver diseases and diabetes. NNMT is involved in a variety of differentiation processes, including naive to mature human embryonic stem cell transition, myogenesis, and epithelial and neuromesenchymal transitions in various cancers. NNMT expression is regulated in a tissue- and context-specific manner rather than by a ubiquitous regulatory pattern.

2.Feature

(1) High purity: NNMTi can obtain high-purity products through refined production processes. High purity means better bioavailability.

(2) Targeting: NNMTi specifically targets the NNMT enzyme and can effectively inhibit its activity, thereby affecting the metabolic pathway of nicotinamide.

(3) Stability: NNMTi has good stability and can maintain its activity and effect under different environments and storage conditions.

(4)Development prospects: Due to the various characteristics of NNMTi, its development has received widespread attention and has broad research and application prospects.

3.Applications

NNMTi (CAS 42464-96-0) is a nicotinamide N-methyltransferase (NNMT) inhibitor, a potent inhibitor (IC50=1.2 μM) of nicotinamide N-methyltransferase (NNMT) that promotes myoblast differentiation. NNMTi selectively binds to NNMT substrate binding site residues. NNMTi promotes myoblast differentiation in vitro and enhances muscle stem cell fusion and regeneration in aged mice. NNMT is a fundamental cytosolic biotransformation enzyme that catalyzes the N-methylation of endogenous and exogenous xenobiotics. Compared with lean diet replacement alone, NNMTi treatment combined with lean diet replacement accelerated improvements in body fat loss, increased whole body lean mass to body weight ratio, reduced liver epididymal white adipose tissue weight, reduced liver fat deposition, and improved liver steatosis. In addition, NNMTi can increase strength and promote the regenerative capacity of aged muscle, which may be useful for the treatment of sarcopenia.

【Frequently Asked Questions】
【1】Q: What is your delivery time?
A: Generally 1~5 days after receiving payment.
【2】Q: Where is your shipping port?
A: Shanghai/Ningbo.
【3】Q: What is the specific form of packaging?
A: Usually our packaging is 1kg/bag and 25kg/barrel. Of course, if you have special requirements, we will also package them according to your requirements.
【4】Q: Can your company accept customization and meet customers' specific specifications?
A: Yes, we can.
【5】Q: Can your company provide samples?
A: Yes, we can, please contact us by phone for specific sample information.