1. Product Overview
A-967079 (CAS 1170613-55-4), systematically named (1E,3E)-1-(4-fluorophenyl)-2-methyl-1-penten-3-one oxime, is a potent and selective small-molecule antagonist of the transient receptor potential ankyrin 1 (TRPA1) ion channel. With the molecular formula C₁₂H₁₄FNO and a molecular weight of 207.24 g/mol, it appears as a white to light yellow crystalline solid at room temperature. A-967079 inhibits human and rat TRPA1 with IC₅₀ values of 67 nM and 289 nM respectively, shows >1,000-fold selectivity over other TRP channels (TRPV1, TRPM8, TRPV4, etc.), and exhibits good central nervous system (CNS) penetration. It is structurally related to the earlier TRPA1 antagonist AP-18 and is widely used as a pharmacological probe in pain, neuroinflammation, and sensory neuron research. This product is supplied exclusively for laboratory research use and is not intended for clinical, diagnostic, veterinary, food, or cosmetic applications.
2. Key Features and Advantages
• High potency and selectivity: Selective TRPA1 antagonist (IC₅₀ = 67 nM human, 289 nM rat); >1,000-fold selectivity over other TRP channels and >150-fold selectivity over 75 other ion channels, enzymes, and GPCRs.
• CNS-penetrant: Demonstrated brain exposure after systemic administration, enabling in vivo studies of central TRPA1 signaling in pain and neuroinflammation.
• High purity: Typically ≥98% by HPLC (suppliers also offer 99.30%), suitable for electrophysiology, calcium imaging, behavioral assays, and Western blot/qPCR in sensory neuron models.
• Good solubility: Readily soluble in DMSO (≥50 mg/mL; prepare 10–50 mM stock in anhydrous DMSO), also soluble in ethanol and methanol; practically insoluble in water.
• Consistent batch quality with supporting analytical data (HPLC, LC-MS, NMR) available from qualified suppliers; referenced in Mol. Pain. 2010 and multiple Nature/Cell papers for TRPA1 modulation in osteoarthritis and inflammatory pain models.
• Stable under recommended storage; light-sensitive oxime derivative that maintains activity when protected from light and moisture.
3. Main Applications
• Pain and nociception research: Evaluating TRPA1 blockade on mechanical/allergic/inflammatory hyperalgesia in CFA-inflamed rat models, osteoarthritis pain models, and wide dynamic range (WDR) neuronal firing assays.
• Sensory neuron studies: Electrophysiology and calcium imaging in DRG neurons to assess TRPA1-dependent responses to mustard oil (allyl isothiocyanate), cinnamaldehyde, and reactive oxygen species.
• Neuroinflammation and CNS research: Leveraging CNS penetration to study TRPA1 in microglia, astrocytes, and central sensitization mechanisms in migraine, cough, and itch pathways.
• Respiratory and airway research: Investigating TRPA1 antagonist effects on cigarette smoke–induced cough, bronchial hyperreactivity, and epithelial cytokine release.
• General research use as a reference standard or pharmacological probe in ion channel drug discovery programs targeting TRP channels for analgesia and anti-inflammatory indications.
4. Technical Specifications
5. Storage and Handling
• Store the powdered compound sealed with desiccant, protected from light; recommended storage at -20°C (long term 3 years; 4°C acceptable for 2 years). For DMSO stock solutions, prepare fresh or aliquot and store at -80°C (6 months stable) or -20°C (1 month); avoid repeated freeze–thaw cycles. Typical working stocks are 10 mM or 50 mM in anhydrous DMSO, protected from light.
• Before opening, allow the sealed vial to warm to room temperature to prevent moisture condensation. Keep container tightly closed when not in use.
• Handle in a well-ventilated area or fume hood. Use personal protective equipment including safety glasses, nitrile gloves, and a lab coat. Avoid inhalation of dust and contact with skin or eyes. This oxime compound may be light-sensitive; minimize exposure to ambient light during handling.
• Intended exclusively for laboratory research by qualified personnel; not for human or veterinary use, food, drug, or cosmetic applications.
6. Safety Overview
• A-967079 is a research chemical TRPA1 antagonist with limited toxicological data. Treat as potentially hazardous: may cause irritation to eyes, skin, and respiratory system.
• In case of eye contact, rinse immediately with plenty of running water for at least 15 minutes and seek medical advice. In case of skin contact, wash thoroughly with soap and water.
• If inhaled, move the person to fresh air and keep at rest in a position comfortable for breathing. If ingested, rinse mouth and seek medical attention—do not induce vomiting unless directed by medical personnel.
• Use appropriate engineering controls (fume hood) and personal protective equipment (safety goggles, nitrile gloves, lab coat). Avoid generating dust and prolonged light exposure.
• Dispose of in accordance with local regulations for chemical waste. Do not discharge into drains, waterways, or soil.
• For detailed safety information, refer to the applicable Safety Data Sheet (SDS) prior to handling.
