1. Product Overview
1-Decanoyl-2-hydroxy-sn-glycero-3-phosphocholine (CAS: 22248-63-1), also known as 1-Decanoyl-lysoPC (LysoPC 10:0) or 1-Capryl-2-hydroxy-sn-glycero-3-phosphocholine, is a short-chain lysophosphatidylcholine (LPC) derivative containing a decanoyl (C10:0) acyl chain at the sn-1 position and a free hydroxyl at sn-2. Supplied as a white to off-white hygroscopic solid or waxy powder, it is an amphiphilic zwitterionic lipid widely used as a model lysophospholipid in membrane biophysics, phospholipase A₂ (PLA₂) activity assays, lipidomics, and liposome formulation studies. Each lot is accompanied by a CoA including HPLC purity, ¹H NMR, and ³¹P NMR verification.
2. Key Features and Advantages
• High Purity: ≥98% (TLC/HPLC), suitable for sensitive lipid bilayer and enzyme kinetics studies
• Fully Characterized: Confirmed by ¹H NMR, ³¹P NMR, and MS; CoA provided per batch
• Amphiphilic Model Compound: Short C10 acyl chain provides enhanced aqueous solubility compared to long-chain lysoPCs, ideal for monolayer/micelle studies
• Low Residual Solvents: Tight control on residual solvents and moisture per ICH Q3 guidelines
• Flexible Packaging: Available in 5 mg / 10 mg / 25 mg / 50 mg / 100 mg / 250 mg / 500 mg; lyophilized or sealed under N₂ on request
• Batch Traceability: Individual lot number with retained sample for re-test
3. Main Applications
• Membrane & Lipid Research: Model lysophospholipid for studying membrane curvature, packing, and phase behavior in lipid bilayers and monolayers
• Enzymatic Assays: Substrate or reference for phospholipase A₂ (PLA₂) activity assays and lipid hydrolysis mechanism studies
• Liposome / Nanoparticle Formulation: Used as a co-surfactant or minor component in cationic/anionic liposomal drug delivery systems
• Lipidomics & Metabolomics: Serves as an internal standard or calibration reference in LC-MS/MS-based phospholipid quantification
• Cell Signaling Studies: Investigated for lysoPC-mediated effects on endothelial cells, inflammation pathways, and calcium signaling
4. Technical Specifications
5. Storage and Handling
• Recommended Storage: Store at –20 °C or below in a tightly sealed, desiccated container protected from light. For long-term storage (>6 months), store under inert atmosphere (N₂ or Ar) in a desiccator to prevent hydrolysis and oxidation.
• Stability: Stable under dry, frozen conditions. Sensitive to moisture, elevated temperature, and prolonged exposure to aqueous media (may undergo hydrolysis). Hygroscopic — handle quickly after opening.
• Handling Precautions: Use in a low-humidity environment or glove box if possible. Wear nitrile gloves, safety goggles, and a lab coat. Avoid generating dust.
• Reconstitution: Dissolve in dry chloroform / methanol (e.g. 2:1 v/v) for stock solution; for aqueous work, sonicate briefly to form micellar dispersion. Prepare fresh when possible.
• Shelf Life: 24 months from date of manufacture when stored as recommended.
6. Safety Overview
• GHS Classification: GHS07 – Warning (non-hazardous at typical lab use levels; classified as irritant in concentrated form)
• H-Statements: H315 – Causes skin irritation; H319 – Causes serious eye irritation; H335 – May cause respiratory irritation
• P-Statements: P261 – Avoid breathing dust/fumes; P264 – Wash hands thoroughly after handling; P280 – Wear protective gloves/eye/face protection; P305+P351+P338 – IF IN EYES: rinse cautiously with water for several minutes; remove contact lenses if present and easy to do; continue rinsing; P501 – Dispose of contents/container in accordance with local regulations
• First Aid:
• Skin: Remove contaminated clothing, wash with plenty of soap and water. Seek medical attention if irritation develops.
• Eyes: Rinse immediately with flowing water ≥15 min; seek medical advice.
• Inhalation: Move to fresh air; if breathing difficulty occurs, seek medical help.
• Ingestion: Rinse mouth; do NOT induce vomiting. Seek medical advice.
• Transport: Not classified as dangerous goods for transport (non-hazardous per IMDG/IATA/DOT).
• Disposal: Dispose as hazardous chemical/lipid waste in compliance with local and national regulations. Do not discharge into drains.
