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5-CARBOXYFLUORESCEIN DIACETATE

5-CARBOXYFLUORESCEIN DIACETATE structure

CAS No:
79955-27-4
Formula:
C25H16O9
Chemical Name:
5-CARBOXYFLUORESCEIN DIACETATE
Synonyms:
5-Carboxy-di-O-acetylfluorescein, 5-CFDA;5-FAM DA;3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic Acid;5-Carboxyfluorescein diacetate;3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid;5-CFDA [5-Carboxyfluorescein diacetate];5-Carboxyfluorescein diacetate≥ 95% (HPLC);BIOCHEMIKA, FOR FLUORESCENCE, ≥95.0% (HPLC)

Description

5-CFDA(5-Carboxyfluorescein diacetate) is membrane-permeant and can be loaded into cells via incubation; hydrolyzed by intracellular esterases to 5-carboxyfluorescein; used for labeling human intervertebral disk cells in vitro for fluorescence microscopy.

5-CARBOXYFLUORESCEIN DIACETATE Basic Attributes

Molecular Weight：

460.39

Exact Mass：

460.079437

BRN：

68265

DSSTox ID：

DTXSID00229971

Color/Form：

Colorless solid

HScode：

32049010

Characteristics

λmax：

<300nm

PSA：

125.43000

XLogP3：

2.40

Appearance：

Solid

Density：

1.57±0.1 g/cm3(Predicted)

Melting Point：

>250°C

Boiling Point：

693.1±55.0 °C(Predicted)

Flash Point：

241.9±25.0 °C

Refractive Index：

1.702

Water Solubility：

DMSO: soluble

Storage Conditions：

2-8°C

PKA：

6.4(at 25℃)

Critical Temperature & Pressure：

Sealed in dry,Store in freezer, under -20°C

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

hazard class code：

safety instructions：

53-22-26-36/37/39-45

Marks：

Precautionary statement(s)：

P264, P280, P302+P352, P305+P351+P338, P321, P332+P313, P337+P313, P362

Hazard Statements：

45-46-23/24/25-36/37/38

GHS Classification：

Not Classified

Drug Information

MeSH Entry Terms：

5-carboxyfluorescein diacetate

5-CARBOXYFLUORESCEIN DIACETATE Use and Manufacturing

5-Carboxyfluorescein diacetate is used in the synthesis of Rhodamines.

Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein.

Computed Properties

Molecular Weight:460.4
XLogP3:3.2
Hydrogen Bond Donor Count:1
Hydrogen Bond Acceptor Count:9
Rotatable Bond Count:5
Exact Mass:460.07943208
Monoisotopic Mass:460.07943208
Topological Polar Surface Area:125
Heavy Atom Count:34
Complexity:828
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes

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Guide of 5-CARBOXYFLUORESCEIN DIACETATE

Description
Basic Attributes
Characteristics
Safety Information
Drug Information
Use and Manufacturing
Computed Properties
Learn More Other Chemicals

5-CARBOXYFLUORESCEIN DIACETATE

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