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Home > Encyclopedia > 3,3′-Diaminobenzidine tetrahydrochloride

3,3′-Diaminobenzidine tetrahydrochloride

3,3′-Diaminobenzidine tetrahydrochloride structure

3,3′-Diaminobenzidine tetrahydrochloride 

structure
  • CAS No:

    7411-49-6

  • Formula:

    C12H14N4.4ClH

  • Chemical Name:

    3,3′-Diaminobenzidine tetrahydrochloride

  • Synonyms:

    [1,1′-Biphenyl]-3,3′,4,4′-tetramine,hydrochloride (1:4);3,3′,4,4′-Biphenyltetramine,tetrahydrochloride;[1,1′-Biphenyl]-3,3′,4,4′-tetramine,tetrahydrochloride;3,3′-Diammoniumbenzidinium tetrachloride;3,3′-Diaminobenzidine tetrahydrochloride;3,3′-Diaminobenzidine hydrochloride;3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride;3,4,3′,4′-Tetraaminobiphenyl tetrahydrochloride;125937-03-3;623161-88-6

  • Categories:

    Organic Chemistry  >  Amides

Description

3,3'-Diaminobenzidine(DAB) is an organic compound that is both chemically and thermodynamically stable; DAB has been used in immunohistochemical staining of nucleic acids and proteins.

3,3′-Diaminobenzidine tetrahydrochloride Basic Attributes

360.11

358.028564

231-018-9

26I0SJP460

DTXSID7021312

Solid

29215900

Characteristics

104.08000

7.21520

tablets

178-180 °C

481.7ºC at 760 mmHg

282.7ºC

H2O: soluble

2-8°C

Peritoneal-mouse LD50: 330 mg/kg

Flammable; burning produces toxic nitrogen oxides and hydrogen chloride fumes

Safety Information

UN 3316 9/PG 2

3

23/24/25-36/38-39/23/24/25-40-20/21/22-68-45-51/53-42/43-60-61

7-16-24-33-36/37-45-24/25-22-36/37/39-53-61

DV8753000

F,T,Xn,N

Treasury is ventilated, low temperature and dry; stored separately from food materials

Stable under normal temperatures and pressures.

P201-P280-P301 + P312 + P330-P305 + P351 + P338-P308 + P313-P337 + P313

H302-H319-H341-H350

SRP: At the time of review, criteria for land treatment or burial (sanitary landfill) disposal practices are subject to significant revision. Prior to implementing land disposal of waste residue (including waste sludge), consult with environmental regulatory agencies for guidance on acceptable disposal practices.

|Danger|H341 (97.92%): Suspected of causing genetic defects [Warning Germ cell mutagenicity]|P201, P202, P281, P308+P313, P405, and P501|Aggregated GHS information provided by 48 companies from 5 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.|H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral]|P201, P202, P264, P270, P273, P281, P301+P312, P308+P313, P330, P391, P405, and P501|The GHS information provided by 1 company from 1 notification to the ECHA C&L Inventory.|H302 (95.12%): Harmful if swallowed [Warning Acute toxicity, oral]|P201, P202, P264, P270, P280, P281, P301+P312, P305+P351+P338, P308+P313, P330, P337+P313, P405, and P501|Aggregated GHS information provided by 41 companies from 3 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

SRP: At the time of review, criteria for land treatment or burial (sanitary landfill) disposal practices are subject to significant revision. Prior to implementing land disposal of waste residue (including waste sludge), consult with environmental regulatory agencies for guidance on acceptable disposal practices.

Toxicity

highly toxic

LD50 Mouse ip 330 mg/kg

Drug Information

Basic treatment: Establish a patent airway. Suction if necessary. Watch for signs of respiratory insufficiency and assist ventilations if needed. Administer oxygen by nonrebreather mask at 10 to 15 L/min. Monitor for pulmonary edema and treat if necessary ... . Monitor for shock and treat if necessary ... . Anticipate seizures and treat if necessary ... . For eye contamination, flush eyes immediately with water. Irrigate each eye continuously with normal saline during transport ... . Do not use emetics. For ingestion, rinse mouth and administer 5 ml/kg up to 200 ml of water for dilution if the patient can swallow, has a strong gag reflex, and does not drool ... . Cover skin burns with dry sterile dressings after decontamination ... . /Poison A and B/|Advanced treatment: Consider orotracheal or nasotracheal intubation for airway control in the patient who is unconscious, has severe pulmonary edema, or is in respiratory arrest. Positive pressure ventilation techniques with a bag valve mask device may be beneficial. Monitor cardiac rhythm and treat arrhythmias as necessary ... . Start an IV with D5W /SRP: "To keep open", minimal flow rate/. Use lactated Ringer's if signs of hypovolemia are present. Watch for signs of fluid overload. Consider drug therapy for pulmonary edema ... . For hypotension with signs of hypovolemia, administer fluid cautiously. Watch for signs of fluid overload ... . Treat seizures with diazepam (Valium) ... . Use proparacaine hydrochloride to assist eye irrigation ... . /Poison A and B/

3,3′-Diaminobenzidine tetrahydrochloride Use and Manufacturing

Uses

A substrate for peroxidase, and a reagent for the spectrophotometric determination of selenium

Production

(1977) NOT PRODUCED COMMERCIALLY IN US|(1979) NOT PRODUCED COMMERCIALLY IN US

ESSENTIALLY 100% AS A RESEARCH CHEMICAL

[1,1'-Biphenyl]-3,3',4,4'-tetramine, hydrochloride (1:4): ACTIVE

Computed Properties

Molecular Weight:360.1
Hydrogen Bond Donor Count:8
Hydrogen Bond Acceptor Count:4
Rotatable Bond Count:1
Exact Mass:360.025607
Monoisotopic Mass:358.028557
Topological Polar Surface Area:104
Heavy Atom Count:20
Complexity:209
Covalently-Bonded Unit Count:5
Compound Is Canonicalized:Yes

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