There is a formula to estimate the correction as a function of ionic strength that I saw on page 30 of the book Buffer Solutions the Basics, by Beynon and Easterby.
That's just a variant of Debye-Huckel, it works reasonably well for ionic strengths of about 0.1 (perhaps even a bit higher, as usual it depends on what accuracy is required), so would be applicable for 0.1M buffer only.
There is a formula to estimate the correction as a function of ionic strength that I saw on page 30 of the book Buffer Solutions the Basics, by Beynon and Easterby.
That's just a variant of Debye-Huckel, it works reasonably well for ionic strengths of about 0.1 (perhaps even a bit higher, as usual it depends on what accuracy is required), so would be applicable for 0.1M buffer only.
The apparent pKa of TrisH+ will be different at 1.0 M buffer strength than 0.1 M. There is a formula to estimate the correction as a function of ionic strength that I saw on page 30 of the book Buffer Solutions the Basics, by Beynon and Easterby. However, I would not be certain that this formula works at such a high concentration, and I would defer to Borek on that question. Jeffreymoonchop's suggestion is reasonable. EDT: More specifically I would add slightly less volume of water than the final solution volume, add HCl to the correct pH, then bring to the final volume with a small additional portion of water.
The apparent pKa of TrisH+ will be different at 1.0 M buffer strength than 0.1 M. There is a formula to estimate the correction as a function of ionic strength that I saw on page 30 of the book Buffer Solutions the Basics, by Beynon and Easterby. However, I would not be certain that this formula works at such a high concentration, and I would defer to Borek on that question. Jeffreymoonchop's suggestion is reasonable. EDT: More specifically I would add slightly less volume of water than the final solution volume, add HCl to the correct pH, then bring to the final volume with a small additional portion of water.
That's just a variant of Debye-Huckel, it works reasonably well for ionic strengths of about 0.1 (perhaps even a bit higher, as usual it depends on what accuracy is required), so would be applicable for 0.1M buffer only.
That's just a variant of Debye-Huckel, it works reasonably well for ionic strengths of about 0.1 (perhaps even a bit higher, as usual it depends on what accuracy is required), so would be applicable for 0.1M buffer only.
More
VOTE
More
VOTE
More
VOTE
From molarity and volume calculate number of moles of both Tris forms, from HH equation calculate their ratio. Converting to masses is rather trivial.
From molarity and volume calculate number of moles of both Tris forms, from HH equation calculate their ratio. Converting to masses is rather trivial.
More
VOTE