The landlord can try it under the conditions of PBS (pH \u003d 7.4, 0.01m).The amount of EDC and Sulfo-NHS is 10: 1, and the response at room temperature should be enough for more than 10 hours.EDC will decompose in the water phase, and Sulfo-NHS plays a stable effect, so EDC needs to be excessive.You try it first, and then tell me if you use the tube.
The landlord can try it under the conditions of PBS (pH \u003d 7.4, 0.01m).The amount of EDC and Sulfo-NHS is 10: 1, and the response at room temperature should be enough for more than 10 hours.EDC will decompose in the water phase, and Sulfo-NHS plays a stable effect, so EDC needs to be excessive.You try it first, and then tell me if you use the tube.
1. The best condition for the EDC reaction is pH 4.5 (or between 4-6), and the efficiency is greatly reduced under neutral conditions. 2. The reaction time is too short, and it will be much better extended to 12h. 3. room temperature is enough. 4. After reaction, consider using dialysis or pillars to remove the small molecular weight. Good Luck!
1. The best condition for the EDC reaction is pH 4.5 (or between 4-6), and the efficiency is greatly reduced under neutral conditions. 2. The reaction time is too short, and it will be much better extended to 12h. 3. room temperature is enough. 4. After reaction, consider using dialysis or pillars to remove the small molecular weight. Good Luck!
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2. The reaction time is too short, and it will be much better extended to 12h.
3. room temperature is enough.
4. After reaction, consider using dialysis or pillars to remove the small molecular weight.
Good Luck!
2. The reaction time is too short, and it will be much better extended to 12h.
3. room temperature is enough.
4. After reaction, consider using dialysis or pillars to remove the small molecular weight.
Good Luck!
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