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Adjusting PBS with HCl: pH drift after dilution of phosphate buffer
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Julian Mintzis
Adjusting PBS with HCl: pH drift after dilution of phosphate buffer
The differences between PBS and the buffer obtained by mixing $\ce{Na2HPO4}$ and
$\ce{NaH2PO4}$ are in ionic strength ( because of the presence of $\ce{KCl}$ d $\ce{NaCl}$ in PBS) and in the ratio of [acid]/[conjugated base]. We have to signal that the buffer capacity (buffer effectiveness) is maximum when $\ce{pH=pKa}$.
The best way to dilute 10x PBS into 1PBS (let's assume we want to prepare 1L of 1xPBS from 10xPBs) is to begin by diluting 100 mL of 10xPBS solution with 800mL water, then adjusting the pH and finally completig the volume to 1000mL. In doing so, there will be no drift in pH.
The differences between PBS and the buffer obtained by mixing $\ce{Na2HPO4}$ and $\ce{NaH2PO4}$ are in ionic strength ( because of the presence of $\ce{KCl}$ d $\ce{NaCl}$ in PBS) and in the ratio of [acid]/[conjugated base]. We have to signal that the buffer capacity (buffer effectiveness) is maximum when $\ce{pH=pKa}$.The best way to dilute 10x PBS into 1PBS (let's assume we want to prepare 1L of 1xPBS from 10xPBs) is to begin by diluting 100 mL of 10xPBS solution with 800mL water, then adjusting the pH and finally completig the volume to 1000mL. In doing so, there will be no drift in pH.
The differences between PBS and the buffer obtained by mixing $\ce{Na2HPO4}$ and $\ce{NaH2PO4}$ are in ionic strength ( because of the presence of $\ce{KCl}$ d $\ce{NaCl}$ in PBS) and in the ratio of [acid]/[conjugated base]. We have to signal that the buffer capacity (buffer effectiveness) is maximum when $\ce{pH=pKa}$. The best way to dilute 10x PBS into 1PBS (let's assume we want to prepare 1L of 1xPBS from 10xPBs) is to begin by diluting 100 mL of 10xPBS solution with 800mL water, then adjusting the pH and finally completig the volume to 1000mL. In doing so, there will be no drift in pH.
The differences between PBS and the buffer obtained by mixing $\ce{Na2HPO4}$ and $\ce{NaH2PO4}$ are in ionic strength ( because of the presence of $\ce{KCl}$ d $\ce{NaCl}$ in PBS) and in the ratio of [acid]/[conjugated base]. We have to signal that the buffer capacity (buffer effectiveness) is maximum when $\ce{pH=pKa}$.The best way to dilute 10x PBS into 1PBS (let's assume we want to prepare 1L of 1xPBS from 10xPBs) is to begin by diluting 100 mL of 10xPBS solution with 800mL water, then adjusting the pH and finally completig the volume to 1000mL. In doing so, there will be no drift in pH.
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