Anthrone reagent: 0.2 % anthrone in conc. H2SO4 Take 1ml of sample/standard in the test tube. (Use 1ml distilled water in a separate tube as a blank/negative control) Add 3ml of anthrone reagent to all the tubes. Mix thoroughly by vortexing. Close/seal the cap of all tubes, incubate at 90 C for 15 -20 minutes. It will produce greenish colour. Cool down all the samples/tubes at room temperature Take OD at 625 using a spectrophotometer.
Anthrone reagent: 0.2 % anthrone in conc. H2SO4 Take 1ml of sample/standard in the test tube. (Use 1ml distilled water in a separate tube as a blank/negative control) Add 3ml of anthrone reagent to all the tubes. Mix thoroughly by vortexing. Close/seal the cap of all tubes, incubate at 90 C for 15 -20 minutes. It will produce greenish colour. Cool down all the samples/tubes at room temperature Take OD at 625 using a spectrophotometer.
We dissolved Anthrone in 98% concentrated sulfuric acid. For the preparation of the standard curve, sucrose solutions were prepared, we need this carbon source. 2: 1 volume of anthrone reagent was added to each solution and it was kept in a water bath at 100 degrees C for 10 minutes. but we got a dark solution, and not the recurring colors, even changing 3: 1 volume of anthrone and solution do you have a comment for us ????
We dissolved Anthrone in 98% concentrated sulfuric acid. For the preparation of the standard curve, sucrose solutions were prepared, we need this carbon source. 2: 1 volume of anthrone reagent was added to each solution and it was kept in a water bath at 100 degrees C for 10 minutes. but we got a dark solution, and not the recurring colors, even changing 3: 1 volume of anthrone and solution do you have a comment for us ????
Those polymers which are made up of glucose unit can be measured using Anthrone reagent. Find the attached file having protocols for estimation of glycogen from animal muscle and organs
Those polymers which are made up of glucose unit can be measured using Anthrone reagent. Find the attached file having protocols for estimation of glycogen from animal muscle and organs
Anthrone method is not a method for estimating tissue glycogen. It is used for measuring carbohydrates- either free or present in polysaccharides.
Anthrone method is not a method for estimating tissue glycogen. It is used for measuring carbohydrates- either free or present in polysaccharides.
More
VOTE
Anthrone reagent: 0.2 % anthrone in conc. H2SO4
Take 1ml of sample/standard in the test tube. (Use 1ml distilled water in a separate tube as a blank/negative control)
Add 3ml of anthrone reagent to all the tubes. Mix thoroughly by vortexing.
Close/seal the cap of all tubes, incubate at 90 C for 15 -20 minutes. It will produce greenish colour.
Cool down all the samples/tubes at room temperature
Take OD at 625 using a spectrophotometer.
Anthrone reagent: 0.2 % anthrone in conc. H2SO4
Take 1ml of sample/standard in the test tube. (Use 1ml distilled water in a separate tube as a blank/negative control)
Add 3ml of anthrone reagent to all the tubes. Mix thoroughly by vortexing.
Close/seal the cap of all tubes, incubate at 90 C for 15 -20 minutes. It will produce greenish colour.
Cool down all the samples/tubes at room temperature
Take OD at 625 using a spectrophotometer.
More
VOTE
Hello
kindly look at links here may help you
Good Luck
https://resources.novusbio.com/manual/Manual-KA0861-2270416.pdf
http://clinchem.aaccjnls.org/content/clinchem/20/12/1576.full.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3992013/
Hello
kindly look at links here may help you
Good Luck
https://resources.novusbio.com/manual/Manual-KA0861-2270416.pdf
http://clinchem.aaccjnls.org/content/clinchem/20/12/1576.full.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3992013/
More
VOTE
We dissolved Anthrone in 98% concentrated sulfuric acid. For the preparation of the standard curve, sucrose solutions were prepared, we need this carbon source.
2: 1 volume of anthrone reagent was added to each solution and it was kept in a water bath at 100 degrees C for 10 minutes. but we got a dark solution, and not the recurring colors, even changing 3: 1 volume of anthrone and solution
do you have a comment for us ????
We dissolved Anthrone in 98% concentrated sulfuric acid. For the preparation of the standard curve, sucrose solutions were prepared, we need this carbon source.
2: 1 volume of anthrone reagent was added to each solution and it was kept in a water bath at 100 degrees C for 10 minutes. but we got a dark solution, and not the recurring colors, even changing 3: 1 volume of anthrone and solution
do you have a comment for us ????
More
VOTE
@zahir hussain- sir can u please suggest me some protocol for total glycogen estimation?
@zahir hussain- sir can u please suggest me some protocol for total glycogen estimation?
More
VOTE
The anthrone method per se will measure all carbohydrates in a sample, including glycogen, glucose, glycolipids, glycoproteins, etc.
The anthrone method per se will measure all carbohydrates in a sample, including glycogen, glucose, glycolipids, glycoproteins, etc.
More
VOTE
Those polymers which are made up of glucose unit can be measured using Anthrone reagent. Find the attached file having protocols for estimation of glycogen from animal muscle and organs
Those polymers which are made up of glucose unit can be measured using Anthrone reagent. Find the attached file having protocols for estimation of glycogen from animal muscle and organs
More
VOTE