Home > Community > Any advice on how to prepare protein samples for ICP-OES analysis?
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+ Sample preparation
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Melvin Plant

Any advice on how to prepare protein samples for ICP-OES analysis?

Alfred Dumasi  Follow

Thank you @Foodengine Team for your recommendation. It gave very good insight

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Doctor Lee  Follow
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BPS Glass  Follow

Discuss your sample with the person in charge of running the ICP-OES at your school. They should be in the best position to provide guidance.

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Charles A. Veilleux  Follow

@William Letter, thank you for the advice. Unfortunately, the operator only have experience using the equipment with plant extras.
@ Ulrich Bochert, thank you very much for this. I will try it out.

@Bruce Neagle, thank you for your recommendation

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Charles Stuart  Follow

I don't have any special experience with protein digestions but I did quite a lot of digesting for ICP analysis. I think the main reason for digesting your sample is to avoid clogging of the ICP nebulizer. It should be no problem to extract most metals from your protein by any acid digestion (aqua regia, HNO3, HNO3/H2O2, ... /open, microwave, ...). Just remember to filtrate the digestion and to use a standard matching your solution. To be on the save side try different digestions and compare your results. Perhaps you can even find a suitable reference material.
Good luck, Ulrich

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Deborah Bien  Follow

Like an environmental sample digest your sample with ultrapure nitric acid in aqua regia. Use no more than 100 mg of sample for your digestion.

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