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Beta-Sitosterol (Pure Anhydrous) Solubility
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Mark Neidorff
Beta-Sitosterol (Pure Anhydrous) Solubility
What about using something like limolene or related interesting smelling alkenes as a solvent? Citral is another. The class of chemicals is called terpenoids or isoprenoids. They are found in fruit, cosmetics, etc.
What about using something like limolene or related interesting smelling alkenes as a solvent? Citral is another. The class of chemicals is called terpenoids or isoprenoids. They are found in fruit, cosmetics, etc.
is it a powder or crystals? Crush as small as possible before mixing, and sonicate at say 40C. Maybe you could dissolve it in oil instead. olive oil for example, then apply to scalp.
Hey, Thanks for the suggestion!
It's a very fine powder, not crystals.
I don't have access to a sonicator but I can buy one as long as I'm fully certain that it will help solubilize the beta-sitosterol.
Also, I did already try dissolving it in the purest, organic jojoba oil (for which I also have CoAs) and it didn't dissolve at all.
is it a powder or crystals? Crush as small as possible before mixing, and sonicate at say 40C. Maybe you could dissolve it in oil instead. olive oil for example, then apply to scalp.
Hey, Thanks for the suggestion!
It's a very fine powder, not crystals.
I don't have access to a sonicator but I can buy one as long as I'm fully certain that it will help solubilize the beta-sitosterol.
Also, I did already try dissolving it in the purest, organic jojoba oil (for which I also have CoAs) and it didn't dissolve at all.
Because of the structural and solubility similarities of beta-sitosterol and vitamin D3, I would look into how VD3 is formulated for fortification of milk etc. Note that for VD2 (not a typo) solutions are prepared commercially in propylene glycol or sesame oil. Water-insoluble, anti-cancer drugs were/are dissolved in arachis oil for testing on rats.
If you were to get into the manufacturing phase, Note that elution of ortho-phenylphenol from silicone tubing by sulfonated cyclodextrin has been reported.
Because of the structural and solubility similarities of beta-sitosterol and vitamin D3, I would look into how VD3 is formulated for fortification of milk etc. Note that for VD2 (not a typo) solutions are prepared commercially in propylene glycol or sesame oil. Water-insoluble, anti-cancer drugs were/are dissolved in arachis oil for testing on rats.
If you were to get into the manufacturing phase, Note that elution of ortho-phenylphenol from silicone tubing by sulfonated cyclodextrin has been reported.
is it a powder or crystals? Crush as small as possible before mixing, and sonicate at say 40C. Maybe you could dissolve it in oil instead. olive oil for example, then apply to scalp.
is it a powder or crystals? Crush as small as possible before mixing, and sonicate at say 40C. Maybe you could dissolve it in oil instead. olive oil for example, then apply to scalp.
Its very lipophilic so DCM, toluene, EtOAc should be fine. Have you tried to heat the ethanol?
Thanks for the reply rolnor! You always have fantastic advice!
I forgot to mention, that the solvent needs to be relatively skin-safe.
Because we'll be applying the solution to the scalp.
Hence, I can't really use any of these.
Also, heating the ethanol isn't really practical, as the beta-sitosterol will revert back to its insoluble state once the ethanol is at room temperature again.
That's why I'm looking for a skin-safe solvent that can dissolve beta sitosterol at room temperature.
I know, it's extremely difficult.
Could it be that somehow my ethanol isn't 99.8% but more 95-96%. I do have a certificate of analysis for that but I'm starting to doubt everything.
If the ethanol is 95-96% will these remaining ~4% of water interfere with the dissolving of beta-sitosterol?
Its very lipophilic so DCM, toluene, EtOAc should be fine. Have you tried to heat the ethanol?
Thanks for the reply rolnor! You always have fantastic advice!
I forgot to mention, that the solvent needs to be relatively skin-safe.
Because we'll be applying the solution to the scalp.
Hence, I can't really use any of these.
Also, heating the ethanol isn't really practical, as the beta-sitosterol will revert back to its insoluble state once the ethanol is at room temperature again.
That's why I'm looking for a skin-safe solvent that can dissolve beta sitosterol at room temperature.
I know, it's extremely difficult.
Could it be that somehow my ethanol isn't 99.8% but more 95-96%. I do have a certificate of analysis for that but I'm starting to doubt everything.
If the ethanol is 95-96% will these remaining ~4% of water interfere with the dissolving of beta-sitosterol?
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
I just need to find these hypothetical solvents.
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
I just need to find these hypothetical solvents.
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
I just need to find these hypothetical solvents.
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
Thanks for the response.
Just now, I did try to dissolve 0.25% beta-sitosterol into 99.8% ethanol and heated up the ethanol until boiling.
Then used a high-powered immersion blender until it got fully dissolved.
So that worked as expected.
However, what also worked as expected is - I left the solution at room temperature and 4 hours later, I can see all the beta-sitosterol precipitate at the bottom.
So it definitely doesn't work that way.
This is only a temporary boost in solubility and isn't a long-term fix.
But thanks for the suggestions!
You may still have some dissolved even if you see some precipitates. You know for sure that the solution is saturated. Maybe try removing 10ml of the remaining solution and evaporate to dryness to see if there is still some dissolved.
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
I just need to find these hypothetical solvents.
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
Thanks for the response.
Just now, I did try to dissolve 0.25% beta-sitosterol into 99.8% ethanol and heated up the ethanol until boiling.
Then used a high-powered immersion blender until it got fully dissolved.
So that worked as expected.
However, what also worked as expected is - I left the solution at room temperature and 4 hours later, I can see all the beta-sitosterol precipitate at the bottom.
So it definitely doesn't work that way.
This is only a temporary boost in solubility and isn't a long-term fix.
But thanks for the suggestions!
You may still have some dissolved even if you see some precipitates. You know for sure that the solution is saturated. Maybe try removing 10ml of the remaining solution and evaporate to dryness to see if there is still some dissolved.
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
I just need to find these hypothetical solvents.
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
Thanks for the response.
Just now, I did try to dissolve 0.25% beta-sitosterol into 99.8% ethanol and heated up the ethanol until boiling.
Then used a high-powered immersion blender until it got fully dissolved.
So that worked as expected.
However, what also worked as expected is - I left the solution at room temperature and 4 hours later, I can see all the beta-sitosterol precipitate at the bottom.
So it definitely doesn't work that way.
This is only a temporary boost in solubility and isn't a long-term fix.
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
I just need to find these hypothetical solvents.
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
Thanks for the response.
Just now, I did try to dissolve 0.25% beta-sitosterol into 99.8% ethanol and heated up the ethanol until boiling.
Then used a high-powered immersion blender until it got fully dissolved.
So that worked as expected.
However, what also worked as expected is - I left the solution at room temperature and 4 hours later, I can see all the beta-sitosterol precipitate at the bottom.
So it definitely doesn't work that way.
This is only a temporary boost in solubility and isn't a long-term fix.
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Hey, Thanks for the suggestion!
It's a very fine powder, not crystals.
I don't have access to a sonicator but I can buy one as long as I'm fully certain that it will help solubilize the beta-sitosterol.
Also, I did already try dissolving it in the purest, organic jojoba oil (for which I also have CoAs) and it didn't dissolve at all.
Hey, Thanks for the suggestion!
It's a very fine powder, not crystals.
I don't have access to a sonicator but I can buy one as long as I'm fully certain that it will help solubilize the beta-sitosterol.
Also, I did already try dissolving it in the purest, organic jojoba oil (for which I also have CoAs) and it didn't dissolve at all.
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Yeah, it's a part of the E499 food additive.
But it doesn't solubilize in pure base oils. I have a lot of essential oils, maybe I can try them.
I also found that sulfobutyl beta-cyclodextrin helps solbulize cholesterol.
So, it might work for beta-sitosterol as well.
I'm just seeing some conflicting data regarding its skin safety profile.
Yeah, it's a part of the E499 food additive.
But it doesn't solubilize in pure base oils. I have a lot of essential oils, maybe I can try them.
I also found that sulfobutyl beta-cyclodextrin helps solbulize cholesterol.
So, it might work for beta-sitosterol as well.
I'm just seeing some conflicting data regarding its skin safety profile.
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Note that for VD2 (not a typo) solutions are prepared commercially in propylene glycol or sesame oil.
Water-insoluble, anti-cancer drugs were/are dissolved in arachis oil for testing on rats.
If you were to get into the manufacturing phase, Note that elution of ortho-phenylphenol from silicone tubing by sulfonated cyclodextrin has been reported.
Regards,
MOTOBALL
Note that for VD2 (not a typo) solutions are prepared commercially in propylene glycol or sesame oil.
Water-insoluble, anti-cancer drugs were/are dissolved in arachis oil for testing on rats.
If you were to get into the manufacturing phase, Note that elution of ortho-phenylphenol from silicone tubing by sulfonated cyclodextrin has been reported.
Regards,
MOTOBALL
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Thanks for the reply!
Yeah, beta-sitosterol is surprisingly hydrophobic and extremely difficult to dissolve.
These are some great suggestions but I'm looking to achieve a long-term stable solution at room temperature.
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
Mixing it with one solvent and then with another, isn't a problem.
I just need to find these hypothetical solvents.
Thank you so much
Thanks for the reply!
Yeah, beta-sitosterol is surprisingly hydrophobic and extremely difficult to dissolve.
These are some great suggestions but I'm looking to achieve a long-term stable solution at room temperature.
Therefore, any temporary boost in solubility created by heat, sonification, etc. isn't what I'm after.
Mixing it with one solvent and then with another, isn't a problem.
I just need to find these hypothetical solvents.
Thank you so much
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Sometimes dissolving in one solvent and later mixing with another solvent can help.
Sometimes dissolving in one solvent and later mixing with another solvent can help.
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Thanks for the reply rolnor! You always have fantastic advice!
I forgot to mention, that the solvent needs to be relatively skin-safe.
Because we'll be applying the solution to the scalp.
Hence, I can't really use any of these.
Also, heating the ethanol isn't really practical, as the beta-sitosterol will revert back to its insoluble state once the ethanol is at room temperature again.
That's why I'm looking for a skin-safe solvent that can dissolve beta sitosterol at room temperature.
I know, it's extremely difficult.
Could it be that somehow my ethanol isn't 99.8% but more 95-96%. I do have a certificate of analysis for that but I'm starting to doubt everything.
If the ethanol is 95-96% will these remaining ~4% of water interfere with the dissolving of beta-sitosterol?
Thanks!!!
Thanks for the reply rolnor! You always have fantastic advice!
I forgot to mention, that the solvent needs to be relatively skin-safe.
Because we'll be applying the solution to the scalp.
Hence, I can't really use any of these.
Also, heating the ethanol isn't really practical, as the beta-sitosterol will revert back to its insoluble state once the ethanol is at room temperature again.
That's why I'm looking for a skin-safe solvent that can dissolve beta sitosterol at room temperature.
I know, it's extremely difficult.
Could it be that somehow my ethanol isn't 99.8% but more 95-96%. I do have a certificate of analysis for that but I'm starting to doubt everything.
If the ethanol is 95-96% will these remaining ~4% of water interfere with the dissolving of beta-sitosterol?
Thanks!!!
More
VOTE
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
I think you are missing the point - these are not temporary boosts. These are way to speed up the dissolution. Chances are your problem is not that of the solubility being lower than reported, but that of very slow dissolution kinetics. If so, once you dissolve the sitosterol and prepare the saturated solution it should be stable.
Quote
That's where the rolnor's ideas can come handy, as with some luck in the final preparation concentration of solvents he suggested can be low enough to not be a problem.
More
VOTE
You may still have some dissolved even if you see some precipitates. You know for sure that the solution is saturated. Maybe try removing 10ml of the remaining solution and evaporate to dryness to see if there is still some dissolved.
You may still have some dissolved even if you see some precipitates. You know for sure that the solution is saturated. Maybe try removing 10ml of the remaining solution and evaporate to dryness to see if there is still some dissolved.
More
VOTE
Thanks for the response.
Just now, I did try to dissolve 0.25% beta-sitosterol into 99.8% ethanol and heated up the ethanol until boiling.
Then used a high-powered immersion blender until it got fully dissolved.
So that worked as expected.
However, what also worked as expected is - I left the solution at room temperature and 4 hours later, I can see all the beta-sitosterol precipitate at the bottom.
So it definitely doesn't work that way.
This is only a temporary boost in solubility and isn't a long-term fix.
But thanks for the suggestions!
Thanks for the response.
Just now, I did try to dissolve 0.25% beta-sitosterol into 99.8% ethanol and heated up the ethanol until boiling.
Then used a high-powered immersion blender until it got fully dissolved.
So that worked as expected.
However, what also worked as expected is - I left the solution at room temperature and 4 hours later, I can see all the beta-sitosterol precipitate at the bottom.
So it definitely doesn't work that way.
This is only a temporary boost in solubility and isn't a long-term fix.
But thanks for the suggestions!
More
VOTE