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Can anyone help me with fast green/safranin O staining of...
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+ Medical research
+ Biochemistry
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+ Tissue engineering
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Neela Fazl
Can anyone help me with fast green/safranin O staining of...
Down-loaded from musculoskeletal research center, University of Rochester- the enclosed protocol has worked very well for me on micromass chondrogenesis paraffin sections and knee articular cartilage of rat. I do not see why it should not work in your case. Very well written by Matt Hilton. Pl find as an attachment
Down-loaded from musculoskeletal research center, University of Rochester- the enclosed protocol has worked very well for me on micromass chondrogenesis paraffin sections and knee articular cartilage of rat. I do not see why it should not work in your case. Very well written by Matt Hilton. Pl find as an attachment
Hi! Yesterday, I stained the above protocol on my 2D sample. It worked seamlessly. The tip is I permeabilized the culture with 0.1% Triton X 100 in PBS for 20 min before washing and acetic incubation step.
1. Fixe sample as usual. 2. Permeabilize cells with 0.1% Triton X 100/20 min RT 3. Wash x3 with PBS 4. 1% acetic acid 10 seconds 5. 1% Safranin O 5 min 6. wash x2 with PBS 7. observe
Hi! Yesterday, I stained the above protocol on my 2D sample. It worked seamlessly. The tip is I permeabilized the culture with 0.1% Triton X 100 in PBS for 20 min before washing and acetic incubation step.
1. Fixe sample as usual. 2. Permeabilize cells with 0.1% Triton X 100/20 min RT 3. Wash x3 with PBS 4. 1% acetic acid 10 seconds 5. 1% Safranin O 5 min 6. wash x2 with PBS 7. observe
Down-loaded from musculoskeletal research center, University of Rochester- the enclosed protocol has worked very well for me on micromass chondrogenesis paraffin sections and knee articular cartilage of rat. I do not see why it should not work in your case. Very well written by Matt Hilton. Pl find as an attachment
Down-loaded from musculoskeletal research center, University of Rochester- the enclosed protocol has worked very well for me on micromass chondrogenesis paraffin sections and knee articular cartilage of rat. I do not see why it should not work in your case. Very well written by Matt Hilton. Pl find as an attachment
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PFA the video its very well explained.
PFA the video its very well explained.
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Only my worry is you are trying to chondrogenesis in monolayer. Try also micromass to compare.
Only my worry is you are trying to chondrogenesis in monolayer. Try also micromass to compare.
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Hi! Yesterday, I stained the above protocol on my 2D sample.
It worked seamlessly. The tip is I permeabilized the culture with 0.1% Triton X 100 in PBS for 20 min before washing and acetic incubation step.
1. Fixe sample as usual.
2. Permeabilize cells with 0.1% Triton X 100/20 min RT
3. Wash x3 with PBS
4. 1% acetic acid 10 seconds
5. 1% Safranin O 5 min
6. wash x2 with PBS
7. observe
Hi! Yesterday, I stained the above protocol on my 2D sample.
It worked seamlessly. The tip is I permeabilized the culture with 0.1% Triton X 100 in PBS for 20 min before washing and acetic incubation step.
1. Fixe sample as usual.
2. Permeabilize cells with 0.1% Triton X 100/20 min RT
3. Wash x3 with PBS
4. 1% acetic acid 10 seconds
5. 1% Safranin O 5 min
6. wash x2 with PBS
7. observe
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http://www.ihcworld.com/_protocols/special_stains/safranin_o.htm
http://www.ihcworld.com/_protocols/special_stains/safranin_o.htm
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