Home > Community > Could I make DNA conjugation with fluorophore by EDC/NHS coupling?
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+ Dna
+ Bioconjugation
+ Edc
+ Fluorophore
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MR HARRY

Could I make DNA conjugation with fluorophore by EDC/NHS coupling?

Anthony Picco  Follow

Dear Shin Jae Woo,
Yah sure you can.
The -NH2 of DNA base react with flurophore by the use of EDC/NHS coupling., here the point to note that the both reactant are basic, in the condition you need to maintain pH by using suitable buffer.
May i know whats the intention of ts study? 
Good Luck & Regards
R.Selva...

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