Home >
Community >
Does anyone know a lab protocol for sample preparation for lipid...
Upvote
27
Downvote
+ Analytical biochemistry
+ Nutritional biochemistry
+ Sample preparation
Posted by
Ogenetega Omoyibo
Does anyone know a lab protocol for sample preparation for lipid...
I've read a paper about the lipid profile of microalgae named "Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions". May be it will help you.
I've read a paper about the lipid profile of microalgae named "Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions". May be it will help you.
The following procedure will help you... Approximately 1 g of samples was melted in an oven at 40–50 0C to obtain the fat phase. The upper fat phase was removed after centrifugation at 448 g for 4 min, and then dried by adding anhydrous sodium sulphate to remove the moisture from sample. Fat obtained from samples was transferred into 5 ml glass vials. The decanted samples were all frozen at-18 0C until analysis. For the determination of fatty acids composition (lipid profile) of the samples FAMEs were prepared using standard IUPAC, 1979 method 2.301.
The following procedure will help you... Approximately 1 g of samples was melted in an oven at 40–50 0C to obtain the fat phase. The upper fat phase was removed after centrifugation at 448 g for 4 min, and then dried by adding anhydrous sodium sulphate to remove the moisture from sample. Fat obtained from samples was transferred into 5 ml glass vials. The decanted samples were all frozen at-18 0C until analysis. For the determination of fatty acids composition (lipid profile) of the samples FAMEs were prepared using standard IUPAC, 1979 method 2.301.
You have had several good suggestion, but just some words of warning: When PUFA are your main interest, ensure that you add an antioxidant ( for example 0.05% butylated hydroxy-toluene). If you don´t have free fatty acids in your sample, I would suggest to use an alkaline, rather than acid catalyst for the trans-methyaltion, since the double bonds are more prone to oxidation in acidic environment. This could be KOH in MeOH. Good luck
You have had several good suggestion, but just some words of warning: When PUFA are your main interest, ensure that you add an antioxidant ( for example 0.05% butylated hydroxy-toluene). If you don´t have free fatty acids in your sample, I would suggest to use an alkaline, rather than acid catalyst for the trans-methyaltion, since the double bonds are more prone to oxidation in acidic environment. This could be KOH in MeOH. Good luck
I do have research skills as well as have a research article in this. Please refer my article attached below. If you find any doubt arise then you can contact me during your experiment any time. Thank you.
I do have research skills as well as have a research article in this. Please refer my article attached below. If you find any doubt arise then you can contact me during your experiment any time. Thank you.
I've read a paper about the lipid profile of microalgae named "Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions". May be it will help you.
I've read a paper about the lipid profile of microalgae named "Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions". May be it will help you.
More
VOTE
Hej,
I’d suggest to try transesterification with HCl in MeOH followed by liquid extraction with a non-polar solvent like cyclohexane.
Maybe this might help:
http://www.sciencedirect.com/science/article/pii/S0731708507005213
Hej,
I’d suggest to try transesterification with HCl in MeOH followed by liquid extraction with a non-polar solvent like cyclohexane.
Maybe this might help:
http://www.sciencedirect.com/science/article/pii/S0731708507005213
More
VOTE
The following procedure will help you...
Approximately 1 g of samples was melted in an oven at 40–50 0C to obtain the fat phase. The upper fat phase was removed after centrifugation at 448 g for 4 min, and then dried by adding anhydrous sodium sulphate to remove the moisture from sample. Fat obtained from samples was transferred into 5 ml glass vials. The decanted samples were all frozen at-18 0C until analysis. For the determination of fatty acids composition (lipid profile) of the samples FAMEs were prepared using standard IUPAC, 1979 method 2.301.
The following procedure will help you...
Approximately 1 g of samples was melted in an oven at 40–50 0C to obtain the fat phase. The upper fat phase was removed after centrifugation at 448 g for 4 min, and then dried by adding anhydrous sodium sulphate to remove the moisture from sample. Fat obtained from samples was transferred into 5 ml glass vials. The decanted samples were all frozen at-18 0C until analysis. For the determination of fatty acids composition (lipid profile) of the samples FAMEs were prepared using standard IUPAC, 1979 method 2.301.
More
VOTE
You have had several good suggestion, but just some words of warning:
When PUFA are your main interest, ensure that you add an antioxidant ( for example 0.05% butylated hydroxy-toluene). If you don´t have free fatty acids in your sample, I would suggest to use an alkaline, rather than acid catalyst for the trans-methyaltion, since the double bonds are more prone to oxidation in acidic environment. This could be KOH in MeOH. Good luck
You have had several good suggestion, but just some words of warning:
When PUFA are your main interest, ensure that you add an antioxidant ( for example 0.05% butylated hydroxy-toluene). If you don´t have free fatty acids in your sample, I would suggest to use an alkaline, rather than acid catalyst for the trans-methyaltion, since the double bonds are more prone to oxidation in acidic environment. This could be KOH in MeOH. Good luck
More
VOTE
I do have research skills as well as have a research article in this. Please refer my article attached below. If you find any doubt arise then you can contact me during your experiment any time. Thank you.
Article Fatty acids as biomarkers of microalgae
I do have research skills as well as have a research article in this. Please refer my article attached below. If you find any doubt arise then you can contact me during your experiment any time. Thank you.
Article Fatty acids as biomarkers of microalgae
More
VOTE