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+ Ph
+ Glycerol
+ Nuclear magnetic resonance
+ Buffer
+ Small molecules
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Michelle Roy

Does glycerol in protein buffer interfere with NMR studies?

Daniel Minja  Follow

Thank you all for your insights!

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David L. Avery  Follow

C'est possible de donner la méthode d'analyse des protéines s'il vous plaît

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Curt Welch  Follow

Thank you very match

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Bill Welden  Follow

The hydrogens present in glycerol will likely be present in your NMR spectra. However, you should be able to identify which peaks are due to glycerol and be able to track them throughout your study. With any luck they won't overlap with any other peaks you are analyzing. If you are concerned about your protein interacting with the glycerol you could use an internal standard to quantify the amount of glycerol present in your sample based on your NMR spectra.

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Amos Ekeson  Follow

The hydrogens present in glycerol will likely be present in your NMR spectra. However, you should be able to identify which peaks are due to glycerol and be able to track them throughout your study. With any luck they won't overlap with any other peaks you are analyzing. If you are concerned about your protein interacting with the glycerol you could use an internal standard to quantify the amount of glycerol present in your sample based on your NMR spectra.

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Charles Michaud  Follow

Very much chance that glycerol show it's peaks in NMR spectrum. Use of d6 solvent should be the best choice.

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