Home >
Community >
Glucose concentration and HEPES in Jurkat cell culture?
Upvote
27
Downvote
+ Jurkat cells
+ Chemistry
Posted by
Kevin Landgrebe
Glucose concentration and HEPES in Jurkat cell culture?
Hey, I was wondering the same, did you manage to find an answer to the glucose question?
The Hepes seems to be a more stable buffer. A microscopy person told me, they use it as a buffer for their live cell microscopy for up to 48h outside of the incubator.
Hey, I was wondering the same, did you manage to find an answer to the glucose question?
The Hepes seems to be a more stable buffer. A microscopy person told me, they use it as a buffer for their live cell microscopy for up to 48h outside of the incubator.
I ended up adding glucose to the culture in the beginning to reach the 4500 mg/L, but once the cells started growing I saw no big differences in proliferation between the higher and lower (2000 mg/L) cultures. However, probably probably the glucose level will have an effect on the cells functionality in some ways, but I found to clear answer to what amount of glucose would be best (also some conflicting information). If you find a better answer to this, please let me know too!
I ended up adding glucose to the culture in the beginning to reach the 4500 mg/L, but once the cells started growing I saw no big differences in proliferation between the higher and lower (2000 mg/L) cultures. However, probably probably the glucose level will have an effect on the cells functionality in some ways, but I found to clear answer to what amount of glucose would be best (also some conflicting information). If you find a better answer to this, please let me know too!
Hey, I was wondering the same, did you manage to find an answer to the glucose question?
The Hepes seems to be a more stable buffer. A microscopy person told me, they use it as a buffer for their live cell microscopy for up to 48h outside of the incubator.
Hey, I was wondering the same, did you manage to find an answer to the glucose question?
The Hepes seems to be a more stable buffer. A microscopy person told me, they use it as a buffer for their live cell microscopy for up to 48h outside of the incubator.
More
VOTE
I ended up adding glucose to the culture in the beginning to reach the 4500 mg/L, but once the cells started growing I saw no big differences in proliferation between the higher and lower (2000 mg/L) cultures. However, probably probably the glucose level will have an effect on the cells functionality in some ways, but I found to clear answer to what amount of glucose would be best (also some conflicting information). If you find a better answer to this, please let me know too!
I ended up adding glucose to the culture in the beginning to reach the 4500 mg/L, but once the cells started growing I saw no big differences in proliferation between the higher and lower (2000 mg/L) cultures. However, probably probably the glucose level will have an effect on the cells functionality in some ways, but I found to clear answer to what amount of glucose would be best (also some conflicting information). If you find a better answer to this, please let me know too!
More
VOTE