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+ Chemistry

Posted by

Minhsan Nguyen

Help removing DMF

Confidence Timothy Follow

Try the azeotrope with heptane. Made my life easier many times!More

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Helen Appiah Follow

What's the ratio and to what temp & pressure do i set it? .More

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Craig Brown Follow

I assume you’re tritylating the amine, unless my brain isn’t working correctly. Your starting material may be water soluble but there’s almost no way Fmoc-Trityl-lysine prefers water over organic.More

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Biniam Berhane Follow

Aqueous workup with 5% LiCl can help a lot too to remove the last bits of DMF; extract with chloroform, chloroform/isopropanol, or EtOAc. But I recommend speedvac most.More

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EdwardTeach Follow

Just to be clear, for extraction, I would use the the aqueous layer with EtOAc. Then I would rotovap the organic layer?More

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Jesus Medina Follow

I do not have access to a speedvac.More

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Glen James Follow

I would like to not heat it over to 50 °C for composition purposes.More

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John Crooks Follow

Thank you for your feedback it is very helpful!More

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Dev Das Follow

I add some toluene to the flask and remove with the rotovap. The toluene forms a low boiling azeotrope with DMF. Edit: just saw that you tried that.More

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Miguel Corazao Follow

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John Sammartino Follow

DMSO will work but then the problem will be removing DMSO.More

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Alex Becker Follow

What your current workup?More

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Edward Hart Follow

I edited my post just recently, I should've been more clear from the start.More

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David Miller Follow

Try the azeotrope with heptane. Made my life easier many times!More

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Haroon Lakho Follow

What's the ratio and to what temp & pressure do i set it? .More

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Camille Mayers Follow

What your current workup?More

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Jason Shearin Follow

I edited my post just recently, I should've been more clear from the start.More

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Andy Parker Follow

Maybe lyophilization could work.More

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Drew Siciliano Follow

Thank you!More

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Jeniffer Stapher-Thomas Follow

Our vacuum pumps are pretty good!More

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Jalen Collins Follow

I will look more into lyophilization!More

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Chris Gold Follow

I add some toluene to the flask and remove with the rotovap. The toluene forms a low boiling azeotrope with DMF. Edit: just saw that you tried that.More

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David Cislo Follow

Aqueous workup with 5% LiCl can help a lot too to remove the last bits of DMF; extract with chloroform, chloroform/isopropanol, or EtOAc. But I recommend speedvac most.More

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Finn Murray Follow

Thank you for your feedback it is very helpful!More

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Farah Chaudhry Follow

I do not have access to a speedvac.More

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JB Northwest Follow

I would like to not heat it over to 50 °C for composition purposes.More

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BPS Glass Follow

I add some toluene to the flask and remove with the rotovap. The toluene forms a low boiling azeotrope with DMF. Edit: just saw that you tried that.More

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Chen Tang Follow

Aqueous workup with 5% LiCl can help a lot too to remove the last bits of DMF; extract with chloroform, chloroform/isopropanol, or EtOAc. But I recommend speedvac most.More

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