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Hi, does anyone have an idea on how to purify a molecule with free...
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+ Inorganic chemistry
+ Chemical reaction engineering
+ Inorganic chemicals
+ Thiols
Posted by
Adi Pantilimon
Hi, does anyone have an idea on how to purify a molecule with free...
I need the free thiol form, and actually I tried adding 2-mercaptoethanol for the reduction but again once I'm done I can't separate it from my product in order to get pure thiol.
I need the free thiol form, and actually I tried adding 2-mercaptoethanol for the reduction but again once I'm done I can't separate it from my product in order to get pure thiol.
What is your next step? If it is oxidation, sometimes disulfide can be used instead of thiol, just remember to recalculate molar amounts of the oxidant. In case you need free thiol, try to treat your pure disulfide with phosphines. DTT and TCEP are commonly used, as they are water-soluble and can be washed out. For large scale, you may want to use cheaper reducing agent and in situ protection, if you need to store your thiol.
What is your next step? If it is oxidation, sometimes disulfide can be used instead of thiol, just remember to recalculate molar amounts of the oxidant. In case you need free thiol, try to treat your pure disulfide with phosphines. DTT and TCEP are commonly used, as they are water-soluble and can be washed out. For large scale, you may want to use cheaper reducing agent and in situ protection, if you need to store your thiol.
Why do you want to use 2-mercaptoethanol? It will make cross disulfides with target molecule, therefore complicating your mixture. What is your target thiol? If it's next to impossible to keep it in a reduced form, you may want to redesign your target structure, tuning electronics or introducing steric bulk next to SH.
Why do you want to use 2-mercaptoethanol? It will make cross disulfides with target molecule, therefore complicating your mixture. What is your target thiol? If it's next to impossible to keep it in a reduced form, you may want to redesign your target structure, tuning electronics or introducing steric bulk next to SH.
I need the free thiol form, and actually I tried adding 2-mercaptoethanol for the reduction but again once I'm done I can't separate it from my product in order to get pure thiol.
I need the free thiol form, and actually I tried adding 2-mercaptoethanol for the reduction but again once I'm done I can't separate it from my product in order to get pure thiol.
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Dear
Tanya Sahyoun
Do you need to vacuum distillation under a nitrogen atmosphere.
Best regards.
Dear
Tanya Sahyoun
Do you need to vacuum distillation under a nitrogen atmosphere.
Best regards.
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What is your next step? If it is oxidation, sometimes disulfide can be used instead of thiol, just remember to recalculate molar amounts of the oxidant.
In case you need free thiol, try to treat your pure disulfide with phosphines. DTT and TCEP are commonly used, as they are water-soluble and can be washed out. For large scale, you may want to use cheaper reducing agent and in situ protection, if you need to store your thiol.
What is your next step? If it is oxidation, sometimes disulfide can be used instead of thiol, just remember to recalculate molar amounts of the oxidant.
In case you need free thiol, try to treat your pure disulfide with phosphines. DTT and TCEP are commonly used, as they are water-soluble and can be washed out. For large scale, you may want to use cheaper reducing agent and in situ protection, if you need to store your thiol.
More
VOTE
Why do you want to use 2-mercaptoethanol? It will make cross disulfides with target molecule, therefore complicating your mixture.
What is your target thiol? If it's next to impossible to keep it in a reduced form, you may want to redesign your target structure, tuning electronics or introducing steric bulk next to SH.
Why do you want to use 2-mercaptoethanol? It will make cross disulfides with target molecule, therefore complicating your mixture.
What is your target thiol? If it's next to impossible to keep it in a reduced form, you may want to redesign your target structure, tuning electronics or introducing steric bulk next to SH.
More
VOTE