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How can I control the hyperhydricity in the micropropagation of...
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Mizanur Rahman
How can I control the hyperhydricity in the micropropagation of...
Hello Saba I totally agree with Pathirana and other researchers. You can also use 1.8 mM to 3.6 mM of Potassium Silicate in your media along with other methods to overcome hyperhydricity.
Hello Saba I totally agree with Pathirana and other researchers. You can also use 1.8 mM to 3.6 mM of Potassium Silicate in your media along with other methods to overcome hyperhydricity.
Hyperhydricity is basically caused by the presence of more amount of nitrogen contents in the medium as compared to the requirement by the plant cells and tissues. The simplest way to overcome this problem is to reduce the nitrates from the medium. If the problem still persists, use half strength of the medium. Even, the addition of certain chemicals may also help in this regard.
Hyperhydricity is basically caused by the presence of more amount of nitrogen contents in the medium as compared to the requirement by the plant cells and tissues. The simplest way to overcome this problem is to reduce the nitrates from the medium. If the problem still persists, use half strength of the medium. Even, the addition of certain chemicals may also help in this regard.
This problem can be attributed to gelling agent content, high ammonium concentration, high salt aggromulation, high relative humidity, low light intensity, number of subcultures and type of gelling agent. Therefore, my suggestion is to pay attention to all the mentioned factors, but the best way is to increase the concentration of agar and decrease the concentration of the culture medium (1/2 or 1/4).
This problem can be attributed to gelling agent content, high ammonium concentration, high salt aggromulation, high relative humidity, low light intensity, number of subcultures and type of gelling agent. Therefore, my suggestion is to pay attention to all the mentioned factors, but the best way is to increase the concentration of agar and decrease the concentration of the culture medium (1/2 or 1/4).
You can control it by applying some treatments such as paclobutrazol (PBZ) and calcium prohexadione (PCa), as well as salicylic acid (AS) and benzoic acid (AB) in the spread. These substrates would be very helpful for hyperhidricity control in crop sprouts .
You can control it by applying some treatments such as paclobutrazol (PBZ) and calcium prohexadione (PCa), as well as salicylic acid (AS) and benzoic acid (AB) in the spread. These substrates would be very helpful for hyperhidricity control in crop sprouts .
The basic principle in avoiding hyperhydricity is Improving lignin metabolism in in vitro culture. It could be achieved by nutrient modification/ reduction, gelling agent, pH, and applying certain stress could alleviate the hyperhydric shoots in cultures.
The basic principle in avoiding hyperhydricity is Improving lignin metabolism in in vitro culture. It could be achieved by nutrient modification/ reduction, gelling agent, pH, and applying certain stress could alleviate the hyperhydric shoots in cultures.
Hello Saba, More specific answer could have been given if you indicated the species and methods of culture leading to hyperhydricity. In general, this condition can be overcome by better ventilation of culture vessel, manipulation of solidifying media (even the concentration), more frequent subculture and adding anti hyperhydricity agents such as EM2, gelling agents conyaining pectin or certain polysaccharides extracted from seaweeds. If you are using rich media, try lowering the concentration of major salts (e.g.half MS macro), try replacing ammonium with nitrate. I have noticed that water condensation on culture vessel also contributes to this condition, so vessels that allow better ventilation will help. If your rack gets heated at the bottom (often this happens when there is another set of lights below), you should raise the culture vessel, not allowing it to touch the surface by using a wire mesh for example. If you can maintain the bottom of vessel colder than top, water will condense on the agar, thus reducing hyperhydricity. Good luck! Ranjith
Hello Saba, More specific answer could have been given if you indicated the species and methods of culture leading to hyperhydricity. In general, this condition can be overcome by better ventilation of culture vessel, manipulation of solidifying media (even the concentration), more frequent subculture and adding anti hyperhydricity agents such as EM2, gelling agents conyaining pectin or certain polysaccharides extracted from seaweeds. If you are using rich media, try lowering the concentration of major salts (e.g.half MS macro), try replacing ammonium with nitrate. I have noticed that water condensation on culture vessel also contributes to this condition, so vessels that allow better ventilation will help. If your rack gets heated at the bottom (often this happens when there is another set of lights below), you should raise the culture vessel, not allowing it to touch the surface by using a wire mesh for example. If you can maintain the bottom of vessel colder than top, water will condense on the agar, thus reducing hyperhydricity. Good luck! Ranjith
Hyperhydricity can also be controlled by bottom cooling, which allows water to condense on the medium, the use of cytokinin-meta-topolin (6-(3-Hydroxybenzylamino)purine)</9>, the combination of lower cytokinin and ammonium nitrate in the medium, use of nitrate or glutamine as the sole nitrogen source and decreasing the
Hyperhydricity can also be controlled by bottom cooling, which allows water to condense on the medium, the use of cytokinin-meta-topolin (6-(3-Hydroxybenzylamino)purine), the combination of lower cytokinin and ammonium nitrate in the medium, use of nitrate or glutamine as the sole nitrogen source and decreasing the
Hello Saba
I totally agree with Pathirana and other researchers. You can also use 1.8 mM to 3.6 mM of Potassium Silicate in your media along with other methods to overcome hyperhydricity.
Hello Saba
I totally agree with Pathirana and other researchers. You can also use 1.8 mM to 3.6 mM of Potassium Silicate in your media along with other methods to overcome hyperhydricity.
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Hyperhydricity is basically caused by the presence of more amount of nitrogen contents in the medium as compared to the requirement by the plant cells and tissues. The simplest way to overcome this problem is to reduce the nitrates from the medium.
If the problem still persists, use half strength of the medium. Even, the addition of certain chemicals may also help in this regard.
Hyperhydricity is basically caused by the presence of more amount of nitrogen contents in the medium as compared to the requirement by the plant cells and tissues. The simplest way to overcome this problem is to reduce the nitrates from the medium.
If the problem still persists, use half strength of the medium. Even, the addition of certain chemicals may also help in this regard.
More
VOTE
Please check it.
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/hyperhydricity
Please check it.
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/hyperhydricity
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By the way, please let me know why the high concentration of cytokinin can make a vitrified plant? Thank you so much.
By the way, please let me know why the high concentration of cytokinin can make a vitrified plant? Thank you so much.
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This problem can be attributed to gelling agent content, high ammonium concentration, high salt aggromulation, high relative humidity, low light intensity, number of subcultures and type of gelling agent. Therefore, my suggestion is to pay attention to all the mentioned factors, but the best way is to increase the concentration of agar and decrease the concentration of the culture medium (1/2 or 1/4).
This problem can be attributed to gelling agent content, high ammonium concentration, high salt aggromulation, high relative humidity, low light intensity, number of subcultures and type of gelling agent. Therefore, my suggestion is to pay attention to all the mentioned factors, but the best way is to increase the concentration of agar and decrease the concentration of the culture medium (1/2 or 1/4).
More
VOTE
You can control it by applying some treatments such as paclobutrazol (PBZ) and calcium prohexadione (PCa), as well as salicylic acid (AS) and benzoic acid (AB) in the spread. These substrates would be very helpful for hyperhidricity control in crop sprouts .
You can control it by applying some treatments such as paclobutrazol (PBZ) and calcium prohexadione (PCa), as well as salicylic acid (AS) and benzoic acid (AB) in the spread. These substrates would be very helpful for hyperhidricity control in crop sprouts .
More
VOTE
The basic principle in avoiding hyperhydricity is Improving lignin metabolism in in vitro culture. It could be achieved by nutrient modification/ reduction, gelling agent, pH, and applying certain stress could alleviate the hyperhydric shoots in cultures.
The basic principle in avoiding hyperhydricity is Improving lignin metabolism in in vitro culture. It could be achieved by nutrient modification/ reduction, gelling agent, pH, and applying certain stress could alleviate the hyperhydric shoots in cultures.
More
VOTE
Hello Saba,
More specific answer could have been given if you indicated the species and methods of culture leading to hyperhydricity. In general, this condition can be overcome by better ventilation of culture vessel, manipulation of solidifying media (even the concentration), more frequent subculture and adding anti hyperhydricity agents such as EM2, gelling agents conyaining pectin or certain polysaccharides extracted from seaweeds. If you are using rich media, try lowering the concentration of major salts (e.g.half MS macro), try replacing ammonium with nitrate. I have noticed that water condensation on culture vessel also contributes to this condition, so vessels that allow better ventilation will help. If your rack gets heated at the bottom (often this happens when there is another set of lights below), you should raise the culture vessel, not allowing it to touch the surface by using a wire mesh for example. If you can maintain the bottom of vessel colder than top, water will condense on the agar, thus reducing hyperhydricity.
Good luck!
Ranjith
Hello Saba,
More specific answer could have been given if you indicated the species and methods of culture leading to hyperhydricity. In general, this condition can be overcome by better ventilation of culture vessel, manipulation of solidifying media (even the concentration), more frequent subculture and adding anti hyperhydricity agents such as EM2, gelling agents conyaining pectin or certain polysaccharides extracted from seaweeds. If you are using rich media, try lowering the concentration of major salts (e.g.half MS macro), try replacing ammonium with nitrate. I have noticed that water condensation on culture vessel also contributes to this condition, so vessels that allow better ventilation will help. If your rack gets heated at the bottom (often this happens when there is another set of lights below), you should raise the culture vessel, not allowing it to touch the surface by using a wire mesh for example. If you can maintain the bottom of vessel colder than top, water will condense on the agar, thus reducing hyperhydricity.
Good luck!
Ranjith
More
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More frequent subcultures, using filtered caps, increasing gelling agent and light
More frequent subcultures, using filtered caps, increasing gelling agent and light
More
VOTE
Reduce ammonium nitrate content in medium
Or
Increase agar concentration to solidify
Reduce ammonium nitrate content in medium
Or
Increase agar concentration to solidify
More
VOTE
Hyperhydricity can also be controlled by bottom cooling, which allows water to condense on the medium, the use of cytokinin-meta-topolin (6-(3-Hydroxybenzylamino)purine)</9>, the combination of lower cytokinin and ammonium nitrate in the medium, use of nitrate or glutamine as the sole nitrogen source and decreasing the
Hyperhydricity can also be controlled by bottom cooling, which allows water to condense on the medium, the use of cytokinin-meta-topolin (6-(3-Hydroxybenzylamino)purine), the combination of lower cytokinin and ammonium nitrate in the medium, use of nitrate or glutamine as the sole nitrogen source and decreasing the
More
VOTE
Reduce ammonium nitrate content in medium
Or
Increase agar concentration to solidify
Reduce ammonium nitrate content in medium
Or
Increase agar concentration to solidify
More
VOTE