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How can I dissolve PMSF (Protease inhibitor) in lysis buffer?
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+ Protein purification
+ Protein purification techniques
+ Protein analysis
+ Lysis buffer
+ Protein extraction
+ Protease inhibitors
Posted by
Laurence Cuffe
How can I dissolve PMSF (Protease inhibitor) in lysis buffer?
I was trying to dissolve PMSF in lysis buffer. But due to little water solubility it couldn't get dissolve. Which solvent I should use to dissolve it? I will use this lysis buffer for the protein extraction for WB. I am wondering if ethanol or isopropyl alcohol will interfere with protein extraction or their stability. Please suggest. Thanks chandrashekhar.
