The tube seems to channel the mobile phase to the pump but not after the pump. I have experience that the degasser was damaged but it is usually not possible to check because most tubes are opaque. I believe the bubbles appeared could be present in the mobile phase itself. The only way now it to make sure your mobile phase is free from dissolved gas, especially those containing high portions of water. Some bubbles may be generated from the suction filter head dipping in the mobile phase. You may need to remove and sonicate it. That could help to reduce air bubble generation.
The tube seems to channel the mobile phase to the pump but not after the pump. I have experience that the degasser was damaged but it is usually not possible to check because most tubes are opaque. I believe the bubbles appeared could be present in the mobile phase itself. The only way now it to make sure your mobile phase is free from dissolved gas, especially those containing high portions of water. Some bubbles may be generated from the suction filter head dipping in the mobile phase. You may need to remove and sonicate it. That could help to reduce air bubble generation.
If your problem is to exit the air bubbles, you can wash many time and they will exit but you should know the reason for this problem. Check your degasser, check your mobile phase itself and do a good sonication for it. If the problem is in your degasser and your method is isocratic, you can mix them in an one bottle and sonicate them together then make your HPLC use single line.
If your problem is to exit the air bubbles, you can wash many time and they will exit but you should know the reason for this problem. Check your degasser, check your mobile phase itself and do a good sonication for it. If the problem is in your degasser and your method is isocratic, you can mix them in an one bottle and sonicate them together then make your HPLC use single line.
What do you mean by "because of the degasser damage"?
What do you mean by "because of the degasser damage"?
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The tube seems to channel the mobile phase to the pump but not after the pump. I have experience that the degasser was damaged but it is usually not possible to check because most tubes are opaque. I believe the bubbles appeared could be present in the mobile phase itself. The only way now it to make sure your mobile phase is free from dissolved gas, especially those containing high portions of water. Some bubbles may be generated from the suction filter head dipping in the mobile phase. You may need to remove and sonicate it. That could help to reduce air bubble generation.
The tube seems to channel the mobile phase to the pump but not after the pump. I have experience that the degasser was damaged but it is usually not possible to check because most tubes are opaque. I believe the bubbles appeared could be present in the mobile phase itself. The only way now it to make sure your mobile phase is free from dissolved gas, especially those containing high portions of water. Some bubbles may be generated from the suction filter head dipping in the mobile phase. You may need to remove and sonicate it. That could help to reduce air bubble generation.
More
VOTE
If your problem is to exit the air bubbles, you can wash many time and they will exit but you should know the reason for this problem. Check your degasser, check your mobile phase itself and do a good sonication for it.
If the problem is in your degasser and your method is isocratic, you can mix them in an one bottle and sonicate them together then make your HPLC use single line.
If your problem is to exit the air bubbles, you can wash many time and they will exit but you should know the reason for this problem. Check your degasser, check your mobile phase itself and do a good sonication for it.
If the problem is in your degasser and your method is isocratic, you can mix them in an one bottle and sonicate them together then make your HPLC use single line.
More
VOTE