How can i prepare 50 mM sodium phosphat buffer of pH 7 ?

You should use Henderson-Hasselbach equation:
https://en.wikipedia.org/wiki/Henderson–Hasselbalch_equation
https://www.researchgate.net/file.PostFileLoader.html?id=54f9c88fd11b8b402a8b4586&assetKey=AS%3A273725033779218%401442272541972

Andrew Jackson  Follow Following

You should use Henderson-Hasselbach equation:
https://en.wikipedia.org/wiki/Henderson–Hasselbalch_equation
https://www.researchgate.net/file.PostFileLoader.html?id=54f9c88fd11b8b402a8b4586&assetKey=AS%3A273725033779218%401442272541972

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Adam B Shapiro
Sir, please share reference for this ratio of monobasic and dibasic sodium phosphate to get pH 7 PB 50 mM

Corey Deane  Follow Following

Adam B Shapiro
Sir, please share reference for this ratio of monobasic and dibasic sodium phosphate to get pH 7 PB 50 mM

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https://www.aatbio.com/resources/buffer-preparations-and-recipes/phosphate-buffer-ph-5-8-to-7-4

Bruce A McIntyre  Follow Following

https://www.aatbio.com/resources/buffer-preparations-and-recipes/phosphate-buffer-ph-5-8-to-7-4

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39 parts monobasic and 61 parts dibasic sodium phosphate solutions (by volume, with both solutions at 50 mM) at room temperature will give a pH 7 solution.

Andrew Wrigley  Follow Following

39 parts monobasic and 61 parts dibasic sodium phosphate solutions (by volume, with both solutions at 50 mM) at room temperature will give a pH 7 solution.

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This link might be helpful to make your calculations.

https://www.aatbio.com/resources/buffer-preparations-and-recipes/phosphate-buffer-ph-5-8-to-7-4

Thanks

Danney Barr  Follow Following

This link might be helpful to make your calculations.

https://www.aatbio.com/resources/buffer-preparations-and-recipes/phosphate-buffer-ph-5-8-to-7-4

Thanks

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Adam B Shapiro
Thanks for a swift response

Christopher Imoe  Follow Following

Adam B Shapiro
Thanks for a swift response

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Hi Raghda Abd Elnasser
To prepare 500 mL of 50 mM Phosphate Buffer (pH 7.0)

1. Add 400 mL of distilled water in a suitable container.
2. Add 3.872 g of Na2HPO4•7H2O to the solution.
3. Add 1.457 g of NaH2PO4•H2O to the solution.
4. Adjust solution to final desired pH using HCl or NaOH
5. Make final volume to 500 mL using distilled water. Good luck!

Castle Richard  Follow Following

Hi Raghda Abd Elnasser
To prepare 500 mL of 50 mM Phosphate Buffer (pH 7.0)

1. Add 400 mL of distilled water in a suitable container.
2. Add 3.872 g of Na2HPO4•7H2O to the solution.
3. Add 1.457 g of NaH2PO4•H2O to the solution.
4. Adjust solution to final desired pH using HCl or NaOH
5. Make final volume to 500 mL using distilled water. Good luck!

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39 parts monobasic and 61 parts dibasic sodium phosphate solutions (by volume, with both solutions at 50 mM) at room temperature will give a pH 7 solution.

Dan Klein  Follow Following

39 parts monobasic and 61 parts dibasic sodium phosphate solutions (by volume, with both solutions at 50 mM) at room temperature will give a pH 7 solution.

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To prepare 50mM phosphate buffer with 7.0pH:

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 7.744 g of Na2HPO4•7H2O to the solution.
3. Add 2.913 g of NaH2PO4•H2O to the solution.
4. Adjust solution to final desired pH using HCl or NaOH
5. Add distilled water until volume is 1 L.

Dmitry Dementiev  Follow Following

To prepare 50mM phosphate buffer with 7.0pH:

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 7.744 g of Na2HPO4•7H2O to the solution.
3. Add 2.913 g of NaH2PO4•H2O to the solution.
4. Adjust solution to final desired pH using HCl or NaOH
5. Add distilled water until volume is 1 L.

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Dissolve 0.2651 g of monosodium phosphate and 0.8253 g of disodium phosphate in 100 ml of water to make 50mM phosphate buffer of pH 7.

Alan Goodwin  Follow Following

Dissolve 0.2651 g of monosodium phosphate and 0.8253 g of disodium phosphate in 100 ml of water to make 50mM phosphate buffer of pH 7.

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