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How can I reduce the polydispersity index of a sample ?
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+ Dynamic light scattering
+ Polydispersity index
+ Ultrasonication
+ Chemistry
Posted by
Olayemi Ola
How can I reduce the polydispersity index of a sample ?
I prepared my DLS sample as below;- I sonicated the whole sample at high power then withdrew 10ul of the sample into the cuvette, then added 1ml DI water. I have tried a couple of times with even lower sample volume, but the polydispersity index always ranges between 0.4 - 0.6, and I want it lower. Could the ultrasonication be responsible for this?How do I address this? I really need a monodispersed sample.
