Home >
Community >
How can I visualise DNA by the naked eye?
Upvote
32
Downvote
+ Staining
+ Chemistry
+ Dyes
+ Polymerase chain reaction
Posted by
Aaysha Safare
How can I visualise DNA by the naked eye?
While conducting routine PCR's I have noticed that I can actually see the DNA bands without UV light. This happens when the concentration of the construct is really high, so when I do visualise (i.e. via geldoc) I see a large bright band. the only thing I can think of to explain this, is that I use the FastDigest green buffer (ThermoFisher) to load my samples on to a 0.8% Agarose gel with GelRED to stain. I still observe the characteristic Yellow and blue dye, but my DNA band can be visualised as pink! Could it be possible that the GelRED along with the FastDigest dyes are allowing me to see the DNA?
