Dear Emma, I agree with the recommendation done by Martin de Boer. Only one more recommendation: try to use STR of four-base pair repetition rather than two bp, Once you have your STR analysed then you can estimate the age of the mutation with the software recommended by Davide Risso, for example, or others that are available in your institution.
Dear Emma, I agree with the recommendation done by Martin de Boer. Only one more recommendation: try to use STR of four-base pair repetition rather than two bp, Once you have your STR analysed then you can estimate the age of the mutation with the software recommended by Davide Risso, for example, or others that are available in your institution.
Dear Emma, What you have to do is to look for short tandem repeats (STR) around the mutation of interest. You can do this with teh repeat finder tool: http://tandem.bu.edu/trf/trf.html STR's mutate very frequently due to slippage of the DNA polymerase, depending of the repeat lenght, this mutation rate is about 1% for every generation (25 years). So you have to set up a genescan assay with primers designed around these STR's. Youhave to test all patients with the mutationand probably you will find the same or almost the same lenght of the STR's, in contrast with control samples. Also the distance between the mutation and the STR is playing a role, the recombination rate is depending on the distance, and is about 0,1% per MB per generation.
Dear Emma, What you have to do is to look for short tandem repeats (STR) around the mutation of interest. You can do this with teh repeat finder tool: http://tandem.bu.edu/trf/trf.html STR's mutate very frequently due to slippage of the DNA polymerase, depending of the repeat lenght, this mutation rate is about 1% for every generation (25 years). So you have to set up a genescan assay with primers designed around these STR's. Youhave to test all patients with the mutationand probably you will find the same or almost the same lenght of the STR's, in contrast with control samples. Also the distance between the mutation and the STR is playing a role, the recombination rate is depending on the distance, and is about 0,1% per MB per generation.
Dear Martin and Ana do you think the number of repeats (adjacent to mutation) between patients and healthy candidates can help. If yes how many missing STR repeats (due to slippage) per generation possible?
Dear Martin and Ana do you think the number of repeats (adjacent to mutation) between patients and healthy candidates can help. If yes how many missing STR repeats (due to slippage) per generation possible?
Hi,
I suggest using one of these two software which are very helpful in estimating mutation ages. Here they are:
GENETREE: http://www.stats.ox.ac.uk/~griff/software.html
DMLE+: http://dmle.org/
Hi,
I suggest using one of these two software which are very helpful in estimating mutation ages. Here they are:
GENETREE: http://www.stats.ox.ac.uk/~griff/software.html
DMLE+: http://dmle.org/
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Dear Emma,
I agree with the recommendation done by Martin de Boer. Only one more recommendation: try to use STR of four-base pair repetition rather than two bp, Once you have your STR analysed then you can estimate the age of the mutation with the software recommended by Davide Risso, for example, or others that are available in your institution.
Dear Emma,
I agree with the recommendation done by Martin de Boer. Only one more recommendation: try to use STR of four-base pair repetition rather than two bp, Once you have your STR analysed then you can estimate the age of the mutation with the software recommended by Davide Risso, for example, or others that are available in your institution.
More
VOTE
I agree with Emma not to use two bp repeats, but longer repeats
I agree with Emma not to use two bp repeats, but longer repeats
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VOTE
Dear Emma,
What you have to do is to look for short tandem repeats (STR) around the mutation of interest. You can do this with teh repeat finder tool: http://tandem.bu.edu/trf/trf.html
STR's mutate very frequently due to slippage of the DNA polymerase, depending of the repeat lenght, this mutation rate is about 1% for every generation (25 years). So you have to set up a genescan assay with primers designed around these STR's. Youhave to test all patients with the mutationand probably you will find the same or almost the same lenght of the STR's, in contrast with control samples.
Also the distance between the mutation and the STR is playing a role, the recombination rate is depending on the distance, and is about 0,1% per MB per generation.
Dear Emma,
What you have to do is to look for short tandem repeats (STR) around the mutation of interest. You can do this with teh repeat finder tool: http://tandem.bu.edu/trf/trf.html
STR's mutate very frequently due to slippage of the DNA polymerase, depending of the repeat lenght, this mutation rate is about 1% for every generation (25 years). So you have to set up a genescan assay with primers designed around these STR's. Youhave to test all patients with the mutationand probably you will find the same or almost the same lenght of the STR's, in contrast with control samples.
Also the distance between the mutation and the STR is playing a role, the recombination rate is depending on the distance, and is about 0,1% per MB per generation.
More
VOTE
Dear Martin and Ana do you think the number of repeats (adjacent to mutation) between patients and healthy candidates can help. If yes how many missing STR repeats (due to slippage) per generation possible?
Dear Martin and Ana do you think the number of repeats (adjacent to mutation) between patients and healthy candidates can help. If yes how many missing STR repeats (due to slippage) per generation possible?
More
VOTE