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+ Chemistry
+ Organic chemistry
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Nec Nominare

How do I prepare Sudan III reagent?

Cotondog1116  Follow

My apologies for getting back to you later.. You will be able to recognize the the difference once all the units have been converted. The limiting reagent determines how much product is made. So once it has run out, what is leftover is the excess or extra. So to answer your question, I don’t believe you can just look at a reagent equation and be able to tell the difference. Most will have different units forcing you to solve.

Hope this helps!

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David  Follow

Sudan lll is soluble in Chloroform or toluene(1 mg/ml). It is also soluble in water (<0.1 mg/ml) and ethanol (2 mg/ml). A saturated solution can be prepared in isopropanol.

Preparation of solution:

  1. Prepare a saturated stock solution of Sudan lll in 99% isopropanol.
  2. Dilute 6 ml of the dock solution with 4 ml of water.
  3. Incubate for 5 to 10 minutes.
  4. Filter the solution. This filtrate can be used for several hours.

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Charles Farquhar  Follow

Sudan lll is soluble in Chloroform or toluene(1 mg/ml). It is also soluble in water (<0.1 mg/ml) and ethanol (2 mg/ml). A saturated solution can be prepared in isopropanol.

Preparation of solution:

  1. Prepare a saturated stock solution of Sudan lll in 99% isopropanol.
  2. Dilute 6 ml of the dock solution with 4 ml of water.
  3. Incubate for 5 to 10 minutes.
  4. Filter the solution. This filtrate can be used for several hours.

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