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Martha Kirtley

How do we really interpret some IC50 results?

Blair  Follow

How can you estimate the IC50 using linear regression?

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Barbara Davis  Follow


There is no data and I don't know the specific situation, so I can't explain it.

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Angel Wallace  Follow

Hi,
From Wikipedia:
https://en.wikipedia.org/wiki/IC50#:~:text=IC50%20values%20can%20be%20used%20to%20compare%20the%20potency,increases%20as%20agonist%20concentration%20increases.
"The IC50 is the concentration of an inhibitor where the response (or binding) is reduced by half.IC50 value increases as agonist concentration increases.".
Conversely, IC50 decreases as agonist concentration decreases.
Here is a reference:
Gubler H, Schopfer U, Jacoby E. Theoretical and experimental relationships between percent inhibition and IC50 data observed in high-throughput screening. J Biomol Screen. 2013 Jan;18(1):1-13. doi: 10.1177/1087057112455219

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