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How do you differentiate THP-1 into macrophage with PMA?
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Levffrt
How do you differentiate THP-1 into macrophage with PMA?
THP-1 was cultured under various conditions, but the cells did not adhere. (I used a cell culture treated plate.)Experiments were conducted under the following conditions.1.RPMI1640 + PMA 150nM + 10% FCS 1 day + RPMI1640 rest 1 day2.RPMI1640 + PMA 150nM + 10% FCS 2 day + RPMI1640 rest 1 day3.RPMI1640 + PMA 150nM + 10% FCS 3 day + RPMI1640 rest 2 day4.RPMI 1640 + PMA 300nM +10% FCS 2 day + RPMI1640 rest 1 day5.RPMI 1640 + PMA 300nM +10% FCS 3 day + RPMI1640 rest 1 dayI have searched a lot of papers and research gate questions, but it is difficult to find the proper conditions.What is the best condition for THP-1 to differentiate? And what plate or dish do you use?Also, I'm currently using CD68 as a differentiation marker, please judge if this is appropriate.
