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How do you solve high pressure issues with Ion Chromatography?
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Mad Mann
How do you solve high pressure issues with Ion Chromatography?
Next to the suggestions and questions of the colleagues above, could you be more specific about the type of column, the mobile phase or gradient used and the sample applied?
Next to the suggestions and questions of the colleagues above, could you be more specific about the type of column, the mobile phase or gradient used and the sample applied?
We need a bit more information to help diagnose this issue. What do you mean when you say the KOH level has decreased? What column are you using? What types of samples are you injecting? Have you tried filtering your samples? Do you have in eluent bottle filters on the eluent source line?
We need a bit more information to help diagnose this issue. What do you mean when you say the KOH level has decreased? What column are you using? What types of samples are you injecting? Have you tried filtering your samples? Do you have in eluent bottle filters on the eluent source line?
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
IonChromatography, so it is water-based in solvent is always a bitch, .... you have to be a chemist and a plumber in same time. Free ur system of ur column, check if pump is going fine on low pressure through pipes-capillars, u should have a very low pressure then, insert ur pre-column only to check if that´s the problem, pressure should rise a bit, not much,... pick out ur pre-column and test the main separation-column, ... do that with all components in ur system, .... if it is DIONEX, .... maybe u will have an ion-exchanger, .... this little super-goodies sometimes make troubles, but on high pressure, they will leak. So, maybe it will not be that part. And, after all, rinse with MeOH-enriched-IC-solvent, that takes out the organics more or less and kicks out air in system. I have running an Dionex-Syste over six years and found out that stragedy. More or less, maybe there is a problem with injection (filter that stuff, Eppendorf-Syringefilters or other brands will help to keep ur system free from buggy stuff)
IonChromatography, so it is water-based in solvent is always a bitch, .... you have to be a chemist and a plumber in same time. Free ur system of ur column, check if pump is going fine on low pressure through pipes-capillars, u should have a very low pressure then, insert ur pre-column only to check if that´s the problem, pressure should rise a bit, not much,... pick out ur pre-column and test the main separation-column, ... do that with all components in ur system, .... if it is DIONEX, .... maybe u will have an ion-exchanger, .... this little super-goodies sometimes make troubles, but on high pressure, they will leak. So, maybe it will not be that part. And, after all, rinse with MeOH-enriched-IC-solvent, that takes out the organics more or less and kicks out air in system. I have running an Dionex-Syste over six years and found out that stragedy. More or less, maybe there is a problem with injection (filter that stuff, Eppendorf-Syringefilters or other brands will help to keep ur system free from buggy stuff)
Usually when my IC instruments have high pressure, it is a bubble or a clog due to contamination. Since you rinsed with higher strength eluent, I would guess the problem to not be a clog.
To try to clear bubbles, prime the pump, then turn it on. Start the monitor pump pressure feature and let it go for a few minutes to have a nice line for a starting point. Then, with the pump still running, open each connection long enough to ensure that there is liquid coming through the line, starting with the line out of the eluent. When there is liquid, close the connection. When you have a union, open the upstream side and make sure there is liquid coming out of the upstream line, then open the downstream side to make sure that there is also liquid coming out of the union. Each time you open and close a connection, wait a few minutes and watch the pressure monitor. If it makes its way back up to where it was, move to the next connection. If the pressure goes up but not as high as before, you probably just cleared a bubble.
Check the condition of the PEEK tubing and the ferrule at each connection. If your line looks kinked or your ferrule mashed, you might need to snip the line or replace the ferrule.
Usually when my IC instruments have high pressure, it is a bubble or a clog due to contamination. Since you rinsed with higher strength eluent, I would guess the problem to not be a clog.
To try to clear bubbles, prime the pump, then turn it on. Start the monitor pump pressure feature and let it go for a few minutes to have a nice line for a starting point. Then, with the pump still running, open each connection long enough to ensure that there is liquid coming through the line, starting with the line out of the eluent. When there is liquid, close the connection. When you have a union, open the upstream side and make sure there is liquid coming out of the upstream line, then open the downstream side to make sure that there is also liquid coming out of the union. Each time you open and close a connection, wait a few minutes and watch the pressure monitor. If it makes its way back up to where it was, move to the next connection. If the pressure goes up but not as high as before, you probably just cleared a bubble.
Check the condition of the PEEK tubing and the ferrule at each connection. If your line looks kinked or your ferrule mashed, you might need to snip the line or replace the ferrule.
A few comments: Be sure to check the solvent (mobile phase) pickup frits and any inline filters (pump and before the column) for partial obstructions). These often clog from use of the high salt buffers. Replacement, sometimes monthly, is a normal part of routine maintenance for these systems.
Additionally, you need to respect the fact that over time, the column will often be clogged ("fouled") with sample material that simple flushing with the same buffer will not in fact remove. You may want to use a stronger flush solution to remove any bound material (contact the column supplier for advice as the best solution to use for this purpose will depend on the exact sample types you are running AND the exact column type being used).
Lastly, HPLC columns are consumable items which eventually must be disposed of. When you can no longer regenerate or clean them to restore performance, please dispose of them. Having a few brand new columns on hand for this purpose is a normal thing (columns cost virtually nothing compared to the cost of the instrument and/or your time and materials in trying to regenerate them).
A few comments: Be sure to check the solvent (mobile phase) pickup frits and any inline filters (pump and before the column) for partial obstructions). These often clog from use of the high salt buffers. Replacement, sometimes monthly, is a normal part of routine maintenance for these systems.
Additionally, you need to respect the fact that over time, the column will often be clogged ("fouled") with sample material that simple flushing with the same buffer will not in fact remove. You may want to use a stronger flush solution to remove any bound material (contact the column supplier for advice as the best solution to use for this purpose will depend on the exact sample types you are running AND the exact column type being used).
Lastly, HPLC columns are consumable items which eventually must be disposed of. When you can no longer regenerate or clean them to restore performance, please dispose of them. Having a few brand new columns on hand for this purpose is a normal thing (columns cost virtually nothing compared to the cost of the instrument and/or your time and materials in trying to regenerate them).
Next to the suggestions and questions of the colleagues above, could you be more specific about the type of column, the mobile phase or gradient used and the sample applied?
Next to the suggestions and questions of the colleagues above, could you be more specific about the type of column, the mobile phase or gradient used and the sample applied?
More
VOTE
We need a bit more information to help diagnose this issue. What do you mean when you say the KOH level has decreased? What column are you using? What types of samples are you injecting? Have you tried filtering your samples? Do you have in eluent bottle filters on the eluent source line?
We need a bit more information to help diagnose this issue. What do you mean when you say the KOH level has decreased? What column are you using? What types of samples are you injecting? Have you tried filtering your samples? Do you have in eluent bottle filters on the eluent source line?
More
VOTE
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
More
VOTE
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
Finding high pressure issues in HPLCs is like a detective's work. You remove one component (plumbing unit) at a time and see its effect on the pressure. First of all remove the column from your system. You can start from the pump to the end of the restrictor of the suppressor. First remove the pump line going to the eluent generator. Turn on the pump. Note the pressure, if is fine, move to the next step. Reconnect the pump to the eluent generator and remove the eluent tubing from the injector port. Turn on the pump and note the pressure. If this is fine, reconnect this line back to the injector port, and turn on the pump again. This time you would note the pressure of the tubing at the column inlet. If this is fine too, attach the column. Note the pressure in one direction, and then reverse the column and note the pressure. If there is a significant difference in pressure, chances are that the column inlet is clogged....continue these steps until you reach the suppressor, and hopefully you will find the culprit.
More
VOTE
IonChromatography, so it is water-based in solvent is always a bitch, .... you have to be a chemist and a plumber in same time. Free ur system of ur column, check if pump is going fine on low pressure through pipes-capillars, u should have a very low pressure then, insert ur pre-column only to check if that´s the problem, pressure should rise a bit, not much,... pick out ur pre-column and test the main separation-column, ... do that with all components in ur system, .... if it is DIONEX, .... maybe u will have an ion-exchanger, .... this little super-goodies sometimes make troubles, but on high pressure, they will leak. So, maybe it will not be that part. And, after all, rinse with MeOH-enriched-IC-solvent, that takes out the organics more or less and kicks out air in system.
I have running an Dionex-Syste over six years and found out that stragedy. More or less, maybe there is a problem with injection (filter that stuff, Eppendorf-Syringefilters or other brands will help to keep ur system free from buggy stuff)
Best regards, H
IonChromatography, so it is water-based in solvent is always a bitch, .... you have to be a chemist and a plumber in same time. Free ur system of ur column, check if pump is going fine on low pressure through pipes-capillars, u should have a very low pressure then, insert ur pre-column only to check if that´s the problem, pressure should rise a bit, not much,... pick out ur pre-column and test the main separation-column, ... do that with all components in ur system, .... if it is DIONEX, .... maybe u will have an ion-exchanger, .... this little super-goodies sometimes make troubles, but on high pressure, they will leak. So, maybe it will not be that part. And, after all, rinse with MeOH-enriched-IC-solvent, that takes out the organics more or less and kicks out air in system.
I have running an Dionex-Syste over six years and found out that stragedy. More or less, maybe there is a problem with injection (filter that stuff, Eppendorf-Syringefilters or other brands will help to keep ur system free from buggy stuff)
Best regards, H
More
VOTE
Usually when my IC instruments have high pressure, it is a bubble or a clog due to contamination. Since you rinsed with higher strength eluent, I would guess the problem to not be a clog.
To try to clear bubbles, prime the pump, then turn it on. Start the monitor pump pressure feature and let it go for a few minutes to have a nice line for a starting point. Then, with the pump still running, open each connection long enough to ensure that there is liquid coming through the line, starting with the line out of the eluent. When there is liquid, close the connection. When you have a union, open the upstream side and make sure there is liquid coming out of the upstream line, then open the downstream side to make sure that there is also liquid coming out of the union. Each time you open and close a connection, wait a few minutes and watch the pressure monitor. If it makes its way back up to where it was, move to the next connection. If the pressure goes up but not as high as before, you probably just cleared a bubble.
Check the condition of the PEEK tubing and the ferrule at each connection. If your line looks kinked or your ferrule mashed, you might need to snip the line or replace the ferrule.
Usually when my IC instruments have high pressure, it is a bubble or a clog due to contamination. Since you rinsed with higher strength eluent, I would guess the problem to not be a clog.
To try to clear bubbles, prime the pump, then turn it on. Start the monitor pump pressure feature and let it go for a few minutes to have a nice line for a starting point. Then, with the pump still running, open each connection long enough to ensure that there is liquid coming through the line, starting with the line out of the eluent. When there is liquid, close the connection. When you have a union, open the upstream side and make sure there is liquid coming out of the upstream line, then open the downstream side to make sure that there is also liquid coming out of the union. Each time you open and close a connection, wait a few minutes and watch the pressure monitor. If it makes its way back up to where it was, move to the next connection. If the pressure goes up but not as high as before, you probably just cleared a bubble.
Check the condition of the PEEK tubing and the ferrule at each connection. If your line looks kinked or your ferrule mashed, you might need to snip the line or replace the ferrule.
More
VOTE
A few comments:
Be sure to check the solvent (mobile phase) pickup frits and any inline filters (pump and before the column) for partial obstructions). These often clog from use of the high salt buffers. Replacement, sometimes monthly, is a normal part of routine maintenance for these systems.
Additionally, you need to respect the fact that over time, the column will often be clogged ("fouled") with sample material that simple flushing with the same buffer will not in fact remove. You may want to use a stronger flush solution to remove any bound material (contact the column supplier for advice as the best solution to use for this purpose will depend on the exact sample types you are running AND the exact column type being used).
Lastly, HPLC columns are consumable items which eventually must be disposed of. When you can no longer regenerate or clean them to restore performance, please dispose of them. Having a few brand new columns on hand for this purpose is a normal thing (columns cost virtually nothing compared to the cost of the instrument and/or your time and materials in trying to regenerate them).
A few comments:
Be sure to check the solvent (mobile phase) pickup frits and any inline filters (pump and before the column) for partial obstructions). These often clog from use of the high salt buffers. Replacement, sometimes monthly, is a normal part of routine maintenance for these systems.
Additionally, you need to respect the fact that over time, the column will often be clogged ("fouled") with sample material that simple flushing with the same buffer will not in fact remove. You may want to use a stronger flush solution to remove any bound material (contact the column supplier for advice as the best solution to use for this purpose will depend on the exact sample types you are running AND the exact column type being used).
Lastly, HPLC columns are consumable items which eventually must be disposed of. When you can no longer regenerate or clean them to restore performance, please dispose of them. Having a few brand new columns on hand for this purpose is a normal thing (columns cost virtually nothing compared to the cost of the instrument and/or your time and materials in trying to regenerate them).
More
VOTE