Under the "Recombination and Integration in Pichia " section you can read how recombination happens. In regards to linearisation, it says in the Pichia transformation section:
1. Digest ~5-10 μg of plasmid DNA with either Avr II (191 bp) or Bsp HI (356 bp). Each enzyme cuts once in the GAP promoter region to linearize the vector. Choose the enzyme that does not cut within your gene.
Under the "Recombination and Integration in Pichia " section you can read how recombination happens. In regards to linearisation, it says in the Pichia transformation section:
1. Digest ~5-10 μg of plasmid DNA with either Avr II (191 bp) or Bsp HI (356 bp). Each enzyme cuts once in the GAP promoter region to linearize the vector. Choose the enzyme that does not cut within your gene.
Have you read this manual:
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/pgapz_man.pdf ?
Under the "Recombination and Integration in Pichia " section you can read how recombination happens.
In regards to linearisation, it says in the Pichia transformation section:
1. Digest ~5-10 μg of plasmid DNA with either Avr II (191 bp) or Bsp HI (356 bp). Each enzyme cuts once in the GAP promoter region to linearize the vector. Choose the enzyme that does not cut within your gene.
Hope that helps.
Best
Kristian
Have you read this manual:
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/pgapz_man.pdf ?
Under the "Recombination and Integration in Pichia " section you can read how recombination happens.
In regards to linearisation, it says in the Pichia transformation section:
1. Digest ~5-10 μg of plasmid DNA with either Avr II (191 bp) or Bsp HI (356 bp). Each enzyme cuts once in the GAP promoter region to linearize the vector. Choose the enzyme that does not cut within your gene.
Hope that helps.
Best
Kristian
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