How is the precipitation of phosphate and calcium chloride formulation medium?

I don't know what culture of LZ is?
According to the principle, the amount of calcium chloride is generally not very large.Therefore, a calcium chloride is added dropwise to the phosphate solution, and the precipitate can be eliminated.

There is a configuration of a M9 medium, which is to drop the calcium chloride in a phosphate solution, just added in it, it will produce a white precipitate, and it disappears after the shock, and there is no effect on bacterial growth.

The calcium phosphate precipitate, is it better to use the dihydrogen phosphate

You can separately formulate calcium salt, and finally add the medium to mix. If you want to sterilize, it is also destroyed.

The phosphate is added to the calcium salted container. If the two can be added simultaneously, the dose of calcium chloride can be increased, and the settlement is reduced.

If you are a relatively large system, such as the fermenter, it can be set as follows:
Direct weighing of phosphate added to the medium, after sterilizing, add calcium chloride and other trace elements when the upper can;
Alternatively, the reservoir is separated separately.Generally no impact.In addition, calcium chloride and other trace elements or antibiotics, such as sulfate,, such as sulfate,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,