I think you can first work out the absolute amount of gallic acid (phenolic cpds) in your 0.1 ml sample, that is [2.38 mg]. You have 20 mL of total volume therefore the total absolute amount = 2.38 mg x 20 = [47.6 mg]. To express mg/dry wt = 47.6mg/0.1g = 476 mg/g. Hope it helps.
I think you can first work out the absolute amount of gallic acid (phenolic cpds) in your 0.1 ml sample, that is [2.38 mg]. You have 20 mL of total volume therefore the total absolute amount = 2.38 mg x 20 = [47.6 mg]. To express mg/dry wt = 47.6mg/0.1g = 476 mg/g. Hope it helps.
If it was a sol, for exemple you did leaching or extraction, you have to convert the unity from mg/l to mg/Kg, so usually we use this method: for example (leaching): 100g ->1L the concentration of nitrite is : 2mg/l so you do 2/100 (mg/g), after that you get 0.02*1000 to have 20mg(nitrite)/kg sol
If it was a sol, for exemple you did leaching or extraction, you have to convert the unity from mg/l to mg/Kg, so usually we use this method: for example (leaching): 100g ->1L the concentration of nitrite is : 2mg/l so you do 2/100 (mg/g), after that you get 0.02*1000 to have 20mg(nitrite)/kg sol
to expess in mg/g of dry matter you have to use calibration curve you will have un equation Y=ax+b to calculate the concentration : (c)=Absorbance/a you will find the result following your calibration curve(unit) then you multiply The extraction volume to return to the mg / g of dry matter. I hope that I have helped you.Good continuation and good luck for the rest
to expess in mg/g of dry matter you have to use calibration curve you will have un equation Y=ax+b to calculate the concentration : (c)=Absorbance/a you will find the result following your calibration curve(unit) then you multiply The extraction volume to return to the mg / g of dry matter. I hope that I have helped you.Good continuation and good luck for the rest
One more question, is it ok to obtain a quantity higher than 100 mg/g?
Thank you very much for your answers.
One more question, is it ok to obtain a quantity higher than 100 mg/g?
Thank you very much for your answers.
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I think you can first work out the absolute amount of gallic acid (phenolic cpds) in your 0.1 ml sample, that is [2.38 mg]. You have 20 mL of total volume therefore the total absolute amount = 2.38 mg x 20 = [47.6 mg]. To express mg/dry wt = 47.6mg/0.1g = 476 mg/g. Hope it helps.
I think you can first work out the absolute amount of gallic acid (phenolic cpds) in your 0.1 ml sample, that is [2.38 mg]. You have 20 mL of total volume therefore the total absolute amount = 2.38 mg x 20 = [47.6 mg]. To express mg/dry wt = 47.6mg/0.1g = 476 mg/g. Hope it helps.
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If it was a sol, for exemple you did leaching or extraction, you have to convert the unity from mg/l to mg/Kg, so usually we use this method:
for example (leaching):
100g ->1L
the concentration of nitrite is : 2mg/l
so you do 2/100 (mg/g), after that you get 0.02*1000 to have 20mg(nitrite)/kg sol
If it was a sol, for exemple you did leaching or extraction, you have to convert the unity from mg/l to mg/Kg, so usually we use this method:
for example (leaching):
100g ->1L
the concentration of nitrite is : 2mg/l
so you do 2/100 (mg/g), after that you get 0.02*1000 to have 20mg(nitrite)/kg sol
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[2.38 mg] in 1 ml of sample!
[2.38 mg] in 1 ml of sample!
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If your matrix is very rich in polyphenols , you can find hight content Which may even exceed 100mg/g of dry matter
good continuation
If your matrix is very rich in polyphenols , you can find hight content Which may even exceed 100mg/g of dry matter
good continuation
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to expess in mg/g of dry matter you have to use calibration curve
you will have un equation Y=ax+b
to calculate the concentration : (c)=Absorbance/a
you will find the result following your calibration curve(unit) then you multiply The extraction volume to return to the mg / g of dry matter.
I hope that I have helped you.Good continuation and good luck for the rest
to expess in mg/g of dry matter you have to use calibration curve
you will have un equation Y=ax+b
to calculate the concentration : (c)=Absorbance/a
you will find the result following your calibration curve(unit) then you multiply The extraction volume to return to the mg / g of dry matter.
I hope that I have helped you.Good continuation and good luck for the rest
More
VOTE