Home >
Community >
How to establish a stable transfection within THP-1 monocytes?
Upvote
34
Downvote
+ Transfection
+ Monocytes
+ Vectorization
+ Thp-1
+ Green fluorescent protein
+ Thp-1 cells
+ Receptor
+ Chemistry
Posted by
Kipha Valvoda
How to establish a stable transfection within THP-1 monocytes?
Hello, i am currently trying to overexpress the CSF-1R receptor in the THP-1 cells in order to create a cell line to be used for the testing of CSF-1R inhibitors. In order to achieve this i am unsure what form of vector i should use, I require a GFP to be encoded by the vector along with the sequence of the CSF-1R.
