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How to get transformed E. coli strains using Promega´s pGL3...
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+ Electroporation
+ Bacterial transformation
+ Molecular biology
+ Molecular genetics
Posted by
Julian Mintzis
How to get transformed E. coli strains using Promega´s pGL3...
I respectively cloned two different promoters from human genes on Promega´s pGL3 vector but I can't obtain any E. coli transformants. The transformation with pGL3 basic control vector works pretty well in all E. coli tested strains: .JM109 (recommended by Promega); Top10 and DH5alfa. It seems the E. coli strains just do not accept the plasmid containing human promoters.Transformation methods tested: electroporation and also Calcium chloride competent cells for all strains.Any suggestion?
