Ficoll-Paque can be prepared using Ficoll and sodium diatrizoate. Sodium diatrizoate helps to provide the optimal density and osmolarity necessary for the efficient removal of other cells from mononuclear cells.
To prepare Ficoll-Paque of density 1.077g/ml, you need to add 6.4g Ficoll and 9.9g sodium diatrizoate to 100ml water. Stir to dissolve in solution. Filter sterilize with a 0.22-μm filter and store at 4° C protected from direct light.
Ficoll-Paque can be prepared using Ficoll and sodium diatrizoate. Sodium diatrizoate helps to provide the optimal density and osmolarity necessary for the efficient removal of other cells from mononuclear cells.
To prepare Ficoll-Paque of density 1.077g/ml, you need to add 6.4g Ficoll and 9.9g sodium diatrizoate to 100ml water. Stir to dissolve in solution. Filter sterilize with a 0.22-μm filter and store at 4° C protected from direct light.
Hello Antonio Abatino
Ficoll-Paque can be prepared using Ficoll and sodium diatrizoate. Sodium diatrizoate helps to provide the optimal density and osmolarity necessary for the efficient removal of other cells from mononuclear cells.
To prepare Ficoll-Paque of density 1.077g/ml, you need to add 6.4g Ficoll and 9.9g sodium diatrizoate to 100ml water. Stir to dissolve in solution. Filter sterilize with a 0.22-μm filter and store at 4° C protected from direct light.
Please refer to the link below.
https://patentimages.storage.googleapis.com/4a/ed/ad/c701138611cd46/US4751001.pdf
Best.
Hello Antonio Abatino
Ficoll-Paque can be prepared using Ficoll and sodium diatrizoate. Sodium diatrizoate helps to provide the optimal density and osmolarity necessary for the efficient removal of other cells from mononuclear cells.
To prepare Ficoll-Paque of density 1.077g/ml, you need to add 6.4g Ficoll and 9.9g sodium diatrizoate to 100ml water. Stir to dissolve in solution. Filter sterilize with a 0.22-μm filter and store at 4° C protected from direct light.
Please refer to the link below.
https://patentimages.storage.googleapis.com/4a/ed/ad/c701138611cd46/US4751001.pdf
Best.
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