Petroleum ether parts are generally suitable for positive phase silicone columns. Generally, the process flow rate of the stem and the pillar is slower.The compounds of the cymbal semi -semi -tadpole are separated by the orthopedic silicon glue column.
Petroleum ether parts are generally suitable for positive phase silicone columns. Generally, the process flow rate of the stem and the pillar is slower.The compounds of the cymbal semi -semi -tadpole are separated by the orthopedic silicon glue column.
At that time, I was a petroleum ether part, and the petroleum ether part of the branches and leaves. There were more than 3600 grams of extract. The so -called pigment, you also know that it is not a specific category compound, so I personally think that there is not much common way! In particular, I don't know what the target component you separated! So you'd better find it while experimenting! In fact, as long as the sample is not lost, any means are worth trying! For the "pigment" with a small polarity, especially like chlorophyll in the branches and leaves, when the amount is large, the large hole resin should be able to try it; if the amount is small, you can try MCI or negative! On the other hand, some "pigments" with greater polarity can be adsorbed on silicone when they are divided or separated! Just like sometimes, after the samples are washed away with positive strokes, the silicone on the column will still be dark! Overall, I think that if the pigment is not very serious, there is no need to deliberately deal with pigment, because from extract to monomer, after all, it is a relatively long process. It must be treated with many chromatography methods. In this process, pigment will inevitably have "loss"! Moreover, after all, in the later period of separation, we can also use gels and liquid phases to purify (or "coloring")! Personal opinions are for reference only, I wish everything go well!
At that time, I was a petroleum ether part, and the petroleum ether part of the branches and leaves. There were more than 3600 grams of extract. The so -called pigment, you also know that it is not a specific category compound, so I personally think that there is not much common way! In particular, I don't know what the target component you separated! So you'd better find it while experimenting! In fact, as long as the sample is not lost, any means are worth trying! For the "pigment" with a small polarity, especially like chlorophyll in the branches and leaves, when the amount is large, the large hole resin should be able to try it; if the amount is small, you can try MCI or negative! On the other hand, some "pigments" with greater polarity can be adsorbed on silicone when they are divided or separated! Just like sometimes, after the samples are washed away with positive strokes, the silicone on the column will still be dark! Overall, I think that if the pigment is not very serious, there is no need to deliberately deal with pigment, because from extract to monomer, after all, it is a relatively long process. It must be treated with many chromatography methods. In this process, pigment will inevitably have "loss"! Moreover, after all, in the later period of separation, we can also use gels and liquid phases to purify (or "coloring")! Personal opinions are for reference only, I wish everything go well!
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The so -called pigment, you also know that it is not a specific category compound, so I personally think that there is not much common way! In particular, I don't know what the target component you separated! So you'd better find it while experimenting! In fact, as long as the sample is not lost, any means are worth trying!
For the "pigment" with a small polarity, especially like chlorophyll in the branches and leaves, when the amount is large, the large hole resin should be able to try it; if the amount is small, you can try MCI or negative!
On the other hand, some "pigments" with greater polarity can be adsorbed on silicone when they are divided or separated! Just like sometimes, after the samples are washed away with positive strokes, the silicone on the column will still be dark!
Overall, I think that if the pigment is not very serious, there is no need to deliberately deal with pigment, because from extract to monomer, after all, it is a relatively long process. It must be treated with many chromatography methods. In this process, pigment will inevitably have "loss"! Moreover, after all, in the later period of separation, we can also use gels and liquid phases to purify (or "coloring")!
Personal opinions are for reference only, I wish everything go well!
The so -called pigment, you also know that it is not a specific category compound, so I personally think that there is not much common way! In particular, I don't know what the target component you separated! So you'd better find it while experimenting! In fact, as long as the sample is not lost, any means are worth trying!
For the "pigment" with a small polarity, especially like chlorophyll in the branches and leaves, when the amount is large, the large hole resin should be able to try it; if the amount is small, you can try MCI or negative!
On the other hand, some "pigments" with greater polarity can be adsorbed on silicone when they are divided or separated! Just like sometimes, after the samples are washed away with positive strokes, the silicone on the column will still be dark!
Overall, I think that if the pigment is not very serious, there is no need to deliberately deal with pigment, because from extract to monomer, after all, it is a relatively long process. It must be treated with many chromatography methods. In this process, pigment will inevitably have "loss"! Moreover, after all, in the later period of separation, we can also use gels and liquid phases to purify (or "coloring")!
Personal opinions are for reference only, I wish everything go well!
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