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+ Chemistry
+ Chromatography
Posted by
Juana Mangaoang

HPLC: Peak height calculation

I am trying to implement a small python script to detect peaks and calculate the corresponding area. The y-values are in mAU and x-values in minutes but there are some unit conversions in most HPLC softwares. For example in this image (found here):

HPLC example

The peak at 7.87 min has a height of 329451 but only approximately 20 V in the chromatogram. How can I calculate this height?

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