Is the acetate and phosphate buffer different?

Acetic acid is weak acid, phosphate is medium -intensity, and the cushioning effect of acetic acid is better. The deadline is different

The tip of acetic acid is high, and the phosphate cannot be used for large proportions of organic phase (can be precipitated).
There is also a problem of buffering salt concentration. Generally, there will be no big difference, and the selectivity of individual material will vary.
In addition, I think you can get effective conclusions as long as you do it. Method development will not be smooth. The method of literature can only be used as a reference and cannot be fully believed.

The deadline of the two solutions can be used as a lower wavelength of phosphate.

Acetic acid is weak acid, and phosphate is strong acid. Under the same conditions, the former's buffer ability is greater than the latter.

Acetate buffer is acidic, and phosphate is neutral. It is easy to combine with two -valent ions

Hehe, try it all, do n’t change it if you do n’t do it. What kind of detector do you use? ELSD do not use phosphate.