The nature of MES and MES buffer Chinese name: 2- (n-morpholine (substitution) telulne acid molecular formula: C6H13NO4S Molecular weight: 195.2 g/mol English name: 4-morpholinethanesulfonic Acid Hydrate; 2- (N-Morpholino) ethanesulfonic Acid Hydrate melting point: ＞ 300 ℃ pka \u003d 6.10 (25 ℃) Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [ Cushion range: pH5.5-6.7 123] The ideal of the ideal biological buffer: 1. PKA is located in a neutral range; 2. Well water solution and not easily soluble in other solvents; 3. not easy to chelate salt ions; 5. PKA is not affected by temperature changes; 6 Stable; 7. There is no obvious light absorption in visible light and ultraviolet light bands; 8. Easy synthesis. The PKA value of MES buffer is close to physiological pH, which is used in active amino gel layer analysis columns to separate the macro -phase components of brain microtubin; MES cannot be absorbed when through the cell membrane Liquid is generally used in the culture of plant cells. Robiot \u0026 Reg; MES buffer is made of high -purity, high -quality MES. After rigorous filtering, it can be directly added to the medium. Preparation: When preparing the MES buffer, use NaOH to adjust the pH to the target pH value (pH5.5-6.7). MES is easy to dissolve in water, and it is colorless and transparent. Before the pH is not adjusted, the pH of the MES solution is between 2.5 and 5. At 0 ° C, the concentration of the saturated MES solution is about 0.65m. Save: MES solution can be stored for several months at 2-8 ° C. It is recommended to use a pore diameter 0.2 micron filter membrane to filter sterilization. (Materials containing sulfuric acid roots are not easy to high temperature sterilization.) After MES is high -temperature sterilizing, it will be pale yellow. As for what the light yellow degradation is not clear, the pH will not change significantly.
The nature of MES and MES buffer Chinese name: 2- (n-morpholine (substitution) telulne acid molecular formula: C6H13NO4S Molecular weight: 195.2 g/mol English name: 4-morpholinethanesulfonic Acid Hydrate; 2- (N-Morpholino) ethanesulfonic Acid Hydrate melting point: ＞ 300 ℃ pka \u003d 6.10 (25 ℃) Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [ Cushion range: pH5.5-6.7 123] The ideal of the ideal biological buffer: 1. PKA is located in a neutral range; 2. Well water solution and not easily soluble in other solvents; 3. not easy to chelate salt ions; 5. PKA is not affected by temperature changes; 6 Stable; 7. There is no obvious light absorption in visible light and ultraviolet light bands; 8. Easy synthesis. The PKA value of MES buffer is close to physiological pH, which is used in active amino gel layer analysis columns to separate the macro -phase components of brain microtubin; MES cannot be absorbed when through the cell membrane Liquid is generally used in the culture of plant cells. Robiot \u0026 Reg; MES buffer is made of high -purity, high -quality MES. After rigorous filtering, it can be directly added to the medium. Preparation: When preparing the MES buffer, use NaOH to adjust the pH to the target pH value (pH5.5-6.7). MES is easy to dissolve in water, and it is colorless and transparent. Before the pH is not adjusted, the pH of the MES solution is between 2.5 and 5. At 0 ° C, the concentration of the saturated MES solution is about 0.65m. Save: MES solution can be stored for several months at 2-8 ° C. It is recommended to use a pore diameter 0.2 micron filter membrane to filter sterilization. (Materials containing sulfuric acid roots are not easy to high temperature sterilization.) After MES is high -temperature sterilizing, it will be pale yellow. As for what the light yellow degradation is not clear, the pH will not change significantly. More
Chinese name: 2- (n-morpholine (substitution) telulne acid
molecular formula: C6H13NO4S
Molecular weight: 195.2 g/mol
English name: 4-morpholinethanesulfonic Acid Hydrate;
2- (N-Morpholino) ethanesulfonic Acid Hydrate
melting point: ＞ 300 ℃
pka \u003d 6.10 (25 ℃)
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [ Cushion range: pH5.5-6.7 123] The ideal of the ideal biological buffer: 1. PKA is located in a neutral range; 2. Well water solution and not easily soluble in other solvents; 3. not easy to chelate salt ions; 5. PKA is not affected by temperature changes; 6 Stable; 7. There is no obvious light absorption in visible light and ultraviolet light bands; 8. Easy synthesis. The PKA value of MES buffer is close to physiological pH, which is used in active amino gel layer analysis columns to separate the macro -phase components of brain microtubin; MES cannot be absorbed when through the cell membrane Liquid is generally used in the culture of plant cells. Robiot \u0026 Reg; MES buffer is made of high -purity, high -quality MES. After rigorous filtering, it can be directly added to the medium. Preparation: When preparing the MES buffer, use NaOH to adjust the pH to the target pH value (pH5.5-6.7). MES is easy to dissolve in water, and it is colorless and transparent. Before the pH is not adjusted, the pH of the MES solution is between 2.5 and 5. At 0 ° C, the concentration of the saturated MES solution is about 0.65m. Save: MES solution can be stored for several months at 2-8 ° C. It is recommended to use a pore diameter 0.2 micron filter membrane to filter sterilization. (Materials containing sulfuric acid roots are not easy to high temperature sterilization.) After MES is high -temperature sterilizing, it will be pale yellow. As for what the light yellow degradation is not clear, the pH will not change significantly.
Chinese name: 2- (n-morpholine (substitution) telulne acid
molecular formula: C6H13NO4S
Molecular weight: 195.2 g/mol
English name: 4-morpholinethanesulfonic Acid Hydrate;
2- (N-Morpholino) ethanesulfonic Acid Hydrate
melting point: ＞ 300 ℃
pka \u003d 6.10 (25 ℃)
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [
Cushion range: pH5.5-6.7 [ Cushion range: pH5.5-6.7 123] The ideal of the ideal biological buffer: 1. PKA is located in a neutral range; 2. Well water solution and not easily soluble in other solvents; 3. not easy to chelate salt ions; 5. PKA is not affected by temperature changes; 6 Stable; 7. There is no obvious light absorption in visible light and ultraviolet light bands; 8. Easy synthesis. The PKA value of MES buffer is close to physiological pH, which is used in active amino gel layer analysis columns to separate the macro -phase components of brain microtubin; MES cannot be absorbed when through the cell membrane Liquid is generally used in the culture of plant cells. Robiot \u0026 Reg; MES buffer is made of high -purity, high -quality MES. After rigorous filtering, it can be directly added to the medium. Preparation: When preparing the MES buffer, use NaOH to adjust the pH to the target pH value (pH5.5-6.7). MES is easy to dissolve in water, and it is colorless and transparent. Before the pH is not adjusted, the pH of the MES solution is between 2.5 and 5. At 0 ° C, the concentration of the saturated MES solution is about 0.65m. Save: MES solution can be stored for several months at 2-8 ° C. It is recommended to use a pore diameter 0.2 micron filter membrane to filter sterilization. (Materials containing sulfuric acid roots are not easy to high temperature sterilization.) After MES is high -temperature sterilizing, it will be pale yellow. As for what the light yellow degradation is not clear, the pH will not change significantly. More
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