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+ Biochemistry techniques
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K.L.

Methylene Blue on Spectrophotometry

David Mott  Follow
Actually I realized my mistake and source of confusion after reading your reply and checking the methylene blue absorption spectrum graph that you included.

You're right that my conclusions were wrong, and it was the main source of my confusion. I should have checked the Methylene Blue absorption spectrum graph or atleast graphed the results of our experiment. By doing so, I should have noticed the downward curvature towards the shorter wavelengths and the abrupt rise and fall on the longer wavelength end. The range of wave length not absorbed at the short wavelength end of the visible spectrum is much bigger than the range of wave length not absorbed at the long wavelength end. Now it all makes sense! Ha!

Thank you for the reply!

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Brodie Brickey  Follow
The spectrum that is shown appears to have a λmax near 660 nm.  What am I missing?

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Celia Milton  Follow
It a little hard to pin down what your question is, although I do get the gist, I think you're building the wrong conclusions for your observations. Lets start with a spectrum.  You can see it here, arbitrary absolution units, across a range of wavelengths.  We have a rainbow under the curve, so you can see what we'd call the colors being absorbed.  This spectrum is pretty typical for molecules that absorb visible light.  Maybe you can ask your question again?  telling us exactly where you don't expect an absorbance and why?

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