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Multiplex giving a negative results? why?
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+ Annealing
+ Chemistry
+ Primer
Posted by
Mark Potter
Multiplex giving a negative results? why?
I ve already done a singleplex of a set of primers against its target gene (DNA template) and it is positive giving a band at the expected size, when i do a multiplex by adding 3 other set of primers the result i have is no band.However i tried gradient annealing starting from 4 degrees below the original primer annealing temperature up to 4 more degrees in a pattern of 2 degrees difference.so can you help?
