pH4.5 acetate buffer preparation

Sodium Sodium Sodium 2.99g, 1.68g of ice acetic acid, add water to 1L, pH value 4.5 ± 0.2

You can refer to the pharmacopoeia standard, which is sodium acetate with water

Page 157 of the Appendix 157 of the Pharmacopoeia Pharmacopoeia. This sodium acetate has crystalline water. If it is waterless, he will write it. You will not be 4.5 with the pH value of sodium -free acetate.It should be purified water, or ionic water.

2.99 g, diluted to 1000ml of 1.6ml ice acetic acid with water.

Water -containing, if the average sodium acetate is not specifically pointed out, there is a water -free sodium aquatic acetate

After weighing the measuring, use acetic acid or sodium hydroxide to adjust it, and then the corresponding pH can be used.Don't be so tangled whether it is water.