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+ Polymerization
+ Transmission electron microscopy (tem)
+ Nanoparticles
+ Carbon
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Mike Windham

TEM Grids for Carbon Quantum Dots?

Andie Spez  Follow

Hello Adam Truskewycz
In your case the material and mesh size is less important, important is to make sure the grids are clean before applying your samples. Few hours of vacuum baking is enough. Holey carbon film copper grids may help you. Although, these grids are costly but they are good for doing EFTEM/EELS for carbon containing or carbon based materials. lacey Formvar/Carbon grid will also be a good alternative because it is cheaper. Usually you can find your particles at the edges.
Please see the website for better information. http://www.agarscientific.com/holey-carbon-films.html


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Deuter Hew  Follow

best option is Silicon Dioxide (SiO2) / Monoxide (SiO) Grids, try 300-400 mesh, its great for carbon-based fluorescent nanoparticles: https://www.2spi.com/category/grids-custom-sio2-monoxide/

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David Smith  Follow

I use a carbon-coated Cu grid of 300-400 mesh. First, gently disperse the CDs in isopropanol/methanol/acetone/chloroform/ethanol. Then, drop cast on-grid and dry under filament lamp and further dry for some hours under vacuum with the heat of about 80-90 degrees. Hope it will work.
I think sample preparation is more important than grid selection.

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Mdrokon Uzzaman  Follow

use any grid but use “in-lens” method

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Dewan Nirjhor  Follow

Hello Adam Truskewycz
You can use very diluted sample of carbon dots and drop on leaky carbon film of copper grind of conventional TEM grids. It works well. We had decorated Ag on carbon dots and imaged them in TEM and HRTEM including controls. It works and you may have to play around with the kV definitely.
The paper where we have put such TEM images is:
Article Metal-Free, Graphene Oxide-Based Tunable Soliton and Plasmon...


Also the following may help:
Article TEM characterization of self‐organized CdSe/ZnSe quantum dots

Article TEM Sample Preparation of Patterned Quantum Dots

Article An optimized TEM specimen preparation method of quantum nanostructures

Good luck!

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