If the sample is not filtered, the stigma is blocked; or because the sample is adsorbed, the stigma part is contaminated, and the fillers of the contaminated stigma part gently stir it with a glass stick and pour it.
If the pillar is contaminated, if it is contaminated with the SEPHADEX LH20 with the opposite phase, the method is as follows: water-naoh-naCl aqueous solution (8G NaOH, 30g NaCl, 1000ml of water), water, methanol washing is contaminated by pollution.The pillars.Because the entire pillar is contaminated, the pollutants will not be completely adsorbed on the column (if it is completely adsorbed on the column, the pollutants will only be on the stigma part), so you can wash it down with the appropriate solvents.
If the sample is not filtered, the stigma is blocked; or because the sample is adsorbed, the stigma part is contaminated, and the fillers of the contaminated stigma part gently stir it with a glass stick and pour it.
If the pillar is contaminated, if it is contaminated with the SEPHADEX LH20 with the opposite phase, the method is as follows: water-naoh-naCl aqueous solution (8G NaOH, 30g NaCl, 1000ml of water), water, methanol washing is contaminated by pollution.The pillars.Because the entire pillar is contaminated, the pollutants will not be completely adsorbed on the column (if it is completely adsorbed on the column, the pollutants will only be on the stigma part), so you can wash it down with the appropriate solvents.
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If the pillar is contaminated, if it is contaminated with the SEPHADEX LH20 with the opposite phase, the method is as follows: water-naoh-naCl aqueous solution (8G NaOH, 30g NaCl, 1000ml of water), water, methanol washing is contaminated by pollution.The pillars.Because the entire pillar is contaminated, the pollutants will not be completely adsorbed on the column (if it is completely adsorbed on the column, the pollutants will only be on the stigma part), so you can wash it down with the appropriate solvents.
If the pillar is contaminated, if it is contaminated with the SEPHADEX LH20 with the opposite phase, the method is as follows: water-naoh-naCl aqueous solution (8G NaOH, 30g NaCl, 1000ml of water), water, methanol washing is contaminated by pollution.The pillars.Because the entire pillar is contaminated, the pollutants will not be completely adsorbed on the column (if it is completely adsorbed on the column, the pollutants will only be on the stigma part), so you can wash it down with the appropriate solvents.
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