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The preparation method of Tris-HCL buffer ~
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Mark Pinsker
The preparation method of Tris-HCL buffer ~
Tris-HCl buffer (0.05 mol / L, 25 ° C), Chinese alias: tris (hydroxymethyl) aminomethane; mamartameriol; ammonium tertiarymmonia; trishydroxymethylmethane; Tris, English Name: Tris (HYDROXYMETHYL) AminometHane Preparation of Tris-HCl buffer (0.05 mol / l, 25 ° C): 50ml 0.1 mol / l Trimethylmethylmethhane (TRIS) solution with x ml 0.1 mol / L After mixing hydrochloric acid, add water to 100 ml Use: Tris buffer is not only widely used as solvents of nucleic acids and proteins, but also many important uses. TRIS is used for protein crystals under different pH conditions. The low ion intensity characteristics of the Tris buffer can be used in the formation of intermediate fibers of nematode (C. elegans) core fibers. TRIS is also one of the main components of the protein electrophoretic buffer, which constitutes a cushioning system in electrophoresis buffer, and stabilizes the pH during electrophoresis. In addition, TRIS is also a surfactant, a vulcanization accelerator, and an intermediate of some drugs. Tris is also used as titration standards. As the protein buffer, if the mass spectrometry is required, it is not suitable for TRIS, it is best to change to other mass spectrometers tolerance Specific configuration, reference link: http://wenku.baidu.com/link URL \u003d ... dukgdp2ftld1sxutqtg
Tris-HCl buffer (0.05 mol / L, 25 ° C), Chinese alias: tris (hydroxymethyl) aminomethane; mamartameriol; ammonium tertiarymmonia; trishydroxymethylmethane; Tris, English Name: Tris (HYDROXYMETHYL) AminometHane Preparation of Tris-HCl buffer (0.05 mol / l, 25 ° C): 50ml 0.1 mol / l Trimethylmethylmethhane (TRIS) solution with x ml 0.1 mol / L After mixing hydrochloric acid, add water to 100 ml Use: Tris buffer is not only widely used as solvents of nucleic acids and proteins, but also many important uses. TRIS is used for protein crystals under different pH conditions. The low ion intensity characteristics of the Tris buffer can be used in the formation of intermediate fibers of nematode (C. elegans) core fibers. TRIS is also one of the main components of the protein electrophoretic buffer, which constitutes a cushioning system in electrophoresis buffer, and stabilizes the pH during electrophoresis. In addition, TRIS is also a surfactant, a vulcanization accelerator, and an intermediate of some drugs. Tris is also used as titration standards. As the protein buffer, if the mass spectrometry is required, it is not suitable for TRIS, it is best to change to other mass spectrometers tolerance Specific configuration, reference link: http://wenku.baidu.com/link URL \u003d ... dukgdp2ftld1sxutqtg
Is the main role of the Tris-HCL buffer, that is, the pH is adjusted. However, I personally experienced that the enzyme protein in the buffer is stabilized in the pure water, then the ions should have a forward effect on the maintenance protein three-dimensional structure.
100mm, PH 8.0 formulation: First plus Tris solid, then use dilute hydrochloric acid to adjust the pH, then set it well.Tris solid molecular weight, you look at the bottle.
Is the main role of the Tris-HCL buffer, that is, the pH is adjusted. However, I personally experienced that the enzyme protein in the buffer is stabilized in the pure water, then the ions should have a forward effect on the maintenance protein three-dimensional structure.
100mm, PH 8.0 formulation: First plus Tris solid, then use dilute hydrochloric acid to adjust the pH, then set it well.Tris solid molecular weight, you look at the bottle.
First calculate the amount of tris, you want 0.1m / L, first take the volume you need, you can calculate how much TRIS, then multiply the molecular weight of it, know how much Tris!After that, the pH was transferred to 8 with HCl
First calculate the amount of tris, you want 0.1m / L, first take the volume you need, you can calculate how much TRIS, then multiply the molecular weight of it, know how much Tris!After that, the pH was transferred to 8 with HCl
Preparation of Tris-HCl buffer (0.05 mol / l, 25 ° C):
50ml 0.1 mol / l Trimethylmethylmethhane (TRIS) solution with x ml 0.1 mol / L After mixing hydrochloric acid, add water to 100 ml
Use:
Tris buffer is not only widely used as solvents of nucleic acids and proteins, but also many important uses. TRIS is used for protein crystals under different pH conditions. The low ion intensity characteristics of the Tris buffer can be used in the formation of intermediate fibers of nematode (C. elegans) core fibers. TRIS is also one of the main components of the protein electrophoretic buffer, which constitutes a cushioning system in electrophoresis buffer, and stabilizes the pH during electrophoresis. In addition, TRIS is also a surfactant, a vulcanization accelerator, and an intermediate of some drugs. Tris is also used as titration standards. As the protein buffer, if the mass spectrometry is required, it is not suitable for TRIS, it is best to change to other mass spectrometers tolerance
Specific configuration, reference link: http://wenku.baidu.com/link URL \u003d ... dukgdp2ftld1sxutqtg
Preparation of Tris-HCl buffer (0.05 mol / l, 25 ° C):
50ml 0.1 mol / l Trimethylmethylmethhane (TRIS) solution with x ml 0.1 mol / L After mixing hydrochloric acid, add water to 100 ml
Use:
Tris buffer is not only widely used as solvents of nucleic acids and proteins, but also many important uses. TRIS is used for protein crystals under different pH conditions. The low ion intensity characteristics of the Tris buffer can be used in the formation of intermediate fibers of nematode (C. elegans) core fibers. TRIS is also one of the main components of the protein electrophoretic buffer, which constitutes a cushioning system in electrophoresis buffer, and stabilizes the pH during electrophoresis. In addition, TRIS is also a surfactant, a vulcanization accelerator, and an intermediate of some drugs. Tris is also used as titration standards. As the protein buffer, if the mass spectrometry is required, it is not suitable for TRIS, it is best to change to other mass spectrometers tolerance
Specific configuration, reference link: http://wenku.baidu.com/link URL \u003d ... dukgdp2ftld1sxutqtg
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However, I personally experienced that the enzyme protein in the buffer is stabilized in the pure water, then the ions should have a forward effect on the maintenance protein three-dimensional structure.
100mm, PH 8.0 formulation: First plus Tris solid, then use dilute hydrochloric acid to adjust the pH, then set it well.Tris solid molecular weight, you look at the bottle.
However, I personally experienced that the enzyme protein in the buffer is stabilized in the pure water, then the ions should have a forward effect on the maintenance protein three-dimensional structure.
100mm, PH 8.0 formulation: First plus Tris solid, then use dilute hydrochloric acid to adjust the pH, then set it well.Tris solid molecular weight, you look at the bottle.
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