The Scatchard eqn can be written as $\displaystyle \frac{Y}{[L]}=\frac{n}{K}-\frac{Y}{K}$ with $[L]$ the free ligand concentration and $Y$ the fraction bound, (i.e. $[L]$ bound /total protein) so the x-axis intercept (when $Y/[L]=0$) is $n$ the number of ligands bound, the slope is $-1/K$ and the y-axis intercept is $n/K$. Your plot confirms this equation, the interpretation of intercepts and slope follow from the equation.
(Your plot seems to plot bound concentration not fraction bound so needs correcting with total protein to make $Y$ and this is presumably why $n$ comes out to be a small value)
The Scatchard eqn can be written as $\displaystyle \frac{Y}{[L]}=\frac{n}{K}-\frac{Y}{K}$ with $[L]$ the free ligand concentration and $Y$ the fraction bound, (i.e. $[L]$ bound /total protein) so the x-axis intercept (when $Y/[L]=0$) is $n$ the number of ligands bound, the slope is $-1/K$ and the y-axis intercept is $n/K$. Your plot confirms this equation, the interpretation of intercepts and slope follow from the equation.
(Your plot seems to plot bound concentration not fraction bound so needs correcting with total protein to make $Y$ and this is presumably why $n$ comes out to be a small value)
The Scatchard eqn can be written as $\displaystyle \frac{Y}{[L]}=\frac{n}{K}-\frac{Y}{K}$ with $[L]$ the free ligand concentration and $Y$ the fraction bound, (i.e. $[L]$ bound /total protein) so the x-axis intercept (when $Y/[L]=0$) is $n$ the number of ligands bound, the slope is $-1/K$ and the y-axis intercept is $n/K$. Your plot confirms this equation, the interpretation of intercepts and slope follow from the equation.
(Your plot seems to plot bound concentration not fraction bound so needs correcting with total protein to make $Y$ and this is presumably why $n$ comes out to be a small value)
The Scatchard eqn can be written as $\displaystyle \frac{Y}{[L]}=\frac{n}{K}-\frac{Y}{K}$ with $[L]$ the free ligand concentration and $Y$ the fraction bound, (i.e. $[L]$ bound /total protein) so the x-axis intercept (when $Y/[L]=0$) is $n$ the number of ligands bound, the slope is $-1/K$ and the y-axis intercept is $n/K$. Your plot confirms this equation, the interpretation of intercepts and slope follow from the equation.
(Your plot seems to plot bound concentration not fraction bound so needs correcting with total protein to make $Y$ and this is presumably why $n$ comes out to be a small value)
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